Expression of tyrosine aminotransferase activity in somatic-cell heterokaryons: evidence for negative control of enzyme expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 10 ·1971-10-00 ·Pages 2589-93

Thompson EB, Gelehrter TD

Abstract

Cells of the tyrosine aminotransferase (EC 2.6.1.5)-inducible line, HTC, were fused with those of the BRL-62 line, which contain little or no enzyme and are unresponsive to the usual steroid inducers. Histochemical examination of the resulting heterokaryons within 24 hr of the fusion showed them to have little or no enzyme and to be unresponsive to steroids. These experiments disclose that the observed negative dominance is universal in the fused cells and that such dominance occurs promptly after fusion, when loss of chromosomes has not occurred.

MeSH Terms

Animals Autoradiography Carcinoma, Hepatocellular/enzymology Cell Fusion Cell Line Cell Nucleus/enzymology Enzyme Induction Histocytochemistry Hybrid Cells/enzymology,metabolism Liver Neoplasms Rats Rats, Inbred Strains Staining and Labeling Thymidine/metabolism Tissue Extracts/analysis Tritium Tyrosine Transaminase/analysis

Chemicals

Tissue Extracts Tritium Tyrosine Transaminase Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Thompson E B

Gelehrter T D

References (26)

26 references, click to expand

The expression of genetic information: a study with hybrid animal cells.
J Cell Sci. 1969 Mar;4(2):499-525 PMID: 5817087
Hormone induced modification of the cell surface.
Nature. 1969 Oct 25;224(5217):344-5 PMID: 4390498
Control of specific gene expression examined in synchronized mammalian cells.
Proc Natl Acad Sci U S A. 1969 Jul;63(3):842-9 PMID: 4390641
Synthesis of an enzyme determined by an erythrocyte nucleus in a hybrid cell.
J Cell Sci. 1969 Jul;5(1):121-33 PMID: 5353650
Control of degradation and synthesis of induced tyrosine aminotransferase studied in hepatoma cells in culture.
Nature. 1969 Nov 22;224(5221):806-8 PMID: 4391285
Dexamethasone phosphate-induced synthesis of tyrosine aminotransferase in hepatoma tissue culture cells. Studies of the early phases of induction and of the steroid requirement for maintanance of the induced rate of synthesis.
J Biol Chem. 1970 Mar 25;245(6):1472-8 PMID: 4392629
The relationship between glucocorticoid binding and tyrosine aminotransferase induction in hepatoma tissue culture cells.
Proc Natl Acad Sci U S A. 1970 Mar;65(3):709-15 PMID: 4315614
Regulation of tyrosine alpha-ketoglutarate transaminase in rat liver. 8. Inductions by hydrocortisone and insulin in cultured hepatoma cells.
J Biol Chem. 1970 Nov 10;245(21):5800-5 PMID: 4394356
Relation of steroid structure to enzyme induction in hepatoma tissue culture cells.
J Mol Biol. 1970 Aug 28;52(1):57-74 PMID: 4395012
Superinduction of tyrosine aminotransferase by actinomycin D in rat hepatoma (HTC) cells.
J Mol Biol. 1970 Dec 14;54(2):159-75 PMID: 4395444
Expression of differentiated functions in hepatoma cell hybrids. I. Tyrosine aminotransferase in hepatoma-fibroblast hybrids.
Proc Natl Acad Sci U S A. 1971 Jan;68(1):127-31 PMID: 4395684
A histochemical method for the demonstration of tyrosine aminotransferase in tissue culture cells, and studies of this enzyme in hepatoma tissue culture cells.
J Cell Biol. 1971 Jun;49(3):921-7 PMID: 4103958
Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell culture.
J Mol Biol. 1968 Jul 28;35(2):291-301 PMID: 4398833
Regulation of gene expression in somatic cell hybrids: a review.
In Vitro. 1971 May-Jun;6(6):411-26 PMID: 4950512
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Control of specific gene expression in higher organisms. Expression of mammalian genes may be controlled by repressors acting on the translation of messenger RNA.
Science. 1969 Dec 19;166(3912):1474-80 PMID: 17655040
Induction of tyrosine alpha-ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line.
Proc Natl Acad Sci U S A. 1966 Jul;56(1):296-303 PMID: 4381784
The role of RNA in the hormonal induction of tyrosine aminotransferase in mammalian cells in tissue culture.
J Mol Biol. 1967 Oct 14;29(1):59-76 PMID: 6055337
Tyrosine aminotransferase. Purificaton and characterization.
J Biol Chem. 1967 Sep 25;242(18):3998-4006 PMID: 4964828
Hybridization of a malignant melanoma cell line with L cells in vitro.
Cancer Res. 1967 Nov;27(11):1953-60 PMID: 6073493
Effect of inhibitors of nucleic acid synthesis on steroid-mediated induction of tyrosine aminotransferase in hepatoma cell cultures.
J Mol Biol. 1967 Nov 28;30(1):49-61 PMID: 4383907
Tyrosine transaminase induction in mammalian cells in tissue culture.
Cold Spring Harb Symp Quant Biol. 1966;31:349-60 PMID: 4295324
Protein turnover and the regulation of enzyme levels in rat liver.
Natl Cancer Inst Monogr. 1967 Nov;27:301-14 PMID: 5583771
Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization. II. The level of regulation of 3,4-dihydroxyphenylalanine oxidase.
Proc Natl Acad Sci U S A. 1968 Jul;60(3):894-901 PMID: 5243931
"Superinduction" of tyrosine transaminase in hepatoma cell cultures: differential inhibition of synthesis and turnover by actionomycin D.
Proc Natl Acad Sci U S A. 1968 Sep;61(1):200-6 PMID: 4386986
Metabolic co-operation between biochemically marked mammalian cells in tissue culture.
J Cell Sci. 1969 Mar;4(2):353-67 PMID: 5817085

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.10.2589

Published

1971-10-00

Pages

2589-93

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389474

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.10.2589 PMC Full Text View journal details

Analysis Services

Expression of tyrosine aminotransferase activity in somatic-cell heterokaryons: evidence for negative control of enzyme expression.

Contact

WeChat Official Account