Inhibition of aggregation and differentiation of Dictyostelium discoideum by antibodies against adenosine 3':5'-cyclic monophosphate diesterase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 5 ·1972-05-00 ·Pages 1128-30

Goidl EA, Chassy BM, Love LL, Krichevsky MI

Abstract

A specific antibody directed against adenosine 3':5'-cyclic monophosphate diesterase (c-AMP diesterase; EC 3.1.4.c), which is secreted by the amoeboid slime mold, Dictyostelium discoideum, was prepared in rabbits. Purified gammaglobulin fractions that contain the antibodies inhibited the activity of cAMP diesterase preparations in vitro and interfered with aggregation and development in vivo. While cells that were treated with antibody were unable to aggregate because of the inability to destroy cAMP, they aggregated normally when washed free of antibody.

MeSH Terms

Animals Antibodies Antibody Specificity Antigens Cell Aggregation Cell Differentiation Chemotaxis Cyclic AMP Immune Sera Immunization Myxomycetes/enzymology,immunology Phosphoric Monoester Hydrolases Rabbits gamma-Globulins/pharmacology

Chemicals

Antibodies Antigens Immune Sera gamma-Globulins Cyclic AMP Phosphoric Monoester Hydrolases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Goidl E A

Chassy B M

Love L L

Krichevsky M I

References (10)

10 references, click to expand

The acrasin activity of adenosine-3',5'-cyclic phosphate.
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1152-4 PMID: 4861307
Cyclic 3',5'-adenosine monophosphate phosphodiesterase produced by the slime mold Dictyostelium discoideum.
Science. 1968 Jul 5;161(3836):57-9 PMID: 4298142
The acrasin activity of 3',5'-cyclic nucleotides.
Proc Natl Acad Sci U S A. 1969 Sep;64(1):296-303 PMID: 4312752
Induction of stalk cell differentiation by cyclic AMP in the cellular slime mold Dictyostelium discoideum.
Proc Natl Acad Sci U S A. 1970 Jan;65(1):110-3 PMID: 4313192
Initiation of slime mold aggregation viewed as an instability.
J Theor Biol. 1970 Mar;26(3):399-415 PMID: 5462335
Conflict between positive and negative feedback as an explanation for the initiation of aggregation in slime mould amoebae.
Nature. 1970 Sep 26;227(5265):1365-6 PMID: 5465943
Phosphodiesterase in Dictyostelium discoideum and the chemotactic response to cyclic adenosine monophosphate.
Science. 1972 Mar 3;175(4025):1014-5 PMID: 4333170
Cyclic nucleotide phosphodiesterase in Dictyostelium discoideum: interconversion of two enzyme forms.
Science. 1972 Mar 3;175(4025):1016-8 PMID: 4333171
Properties of acrasin.
Science. 1956 Jun 29;123(3209):1172-3 PMID: 13337336
ENVIRONMENTAL CONTROL OF THE COURSE OF DEVELOPMENT IN DICTYOSTELIUM DISCOIDEUM.
J Gen Microbiol. 1963 Aug;32:195-207 PMID: 14053267

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.5.1128

Published

1972-05-00

Pages

1128-30

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426646

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.5.1128 PMC Full Text View journal details

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Inhibition of aggregation and differentiation of Dictyostelium discoideum by antibodies against adenosine 3':5'-cyclic monophosphate diesterase.

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