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PMID: 4117961 Published · ppublish English Journal Article

Sendai virus-induced transcriptase from infected cells: polypeptides in the transcriptive complex.

Journal of virology ·Vol. 10 ·No. 5 ·1972-11-00 ·Pages 1037-43

Stone HO, Kingsbury DW, Darlington RW

Abstract

A Sendai virus-induced transcriptase-template complex was isolated from the cytoplasm of infected cells by combined sedimentation and isopycnic centrifugation. This transcriptive complex banded at 1.27 g/cm(3) in D(2)O-sucrose gradients. It contained two polypeptides, the viral nucleocapsid structure unit (molecular weight, 60,000) and the largest virion polypeptide (molecular weight, 75,000). The buoyant density, chemical composition, and electron microscopic appearance of the transcriptive complex indicate a structure like that of viral nucleocapsids.

MeSH Terms
Animals Carbon Isotopes Cell-Free System Cells, Cultured Centrifugation, Density Gradient Chick Embryo Cytoplasm/enzymology DNA-Directed RNA Polymerases/isolation & purification,metabolism Deuterium Electrophoresis, Polyacrylamide Gel Genetics, Microbial Lung Microscopy, Electron Molecular Weight Nucleoproteins/analysis Oxides Parainfluenza Virus 1, Human/analysis Peptides/analysis Staining and Labeling Sucrose Templates, Genetic Transcription, Genetic Tritium Viral Proteins/analysis
Chemicals
Carbon Isotopes Nucleoproteins Oxides Peptides Viral Proteins Tritium Sucrose Deuterium DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stone H O
Kingsbury D W
Darlington R W
References (18)
18 references, click to expand
  1. RNA metabolism in the HeLa cell nucleus.
    J Mol Biol. 1966 May;17(1):117-30 PMID: 5961144
  2. Ribonucleic acid nucleotidyl transferase induced in chick fibroblasts after infection with Newcastle disease virus.
    J Gen Virol. 1969 Jun;4(4):565-70 PMID: 5816815
  3. Observations on the structure of the nucleocapsids of some paramyxoviruses.
    J Gen Virol. 1970 Jan;6(1):141-50 PMID: 4313396
  4. Replication of Sendai virus. II. Steps in virus assembly.
    J Virol. 1970 May;5(5):639-50 PMID: 4315961
  5. Ribonucleic acid polymerase induced in cells infected with Sendai virus.
    J Virol. 1970 Jun;5(6):663-71 PMID: 4317110
  6. Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus.
    J Virol. 1970 Nov;6(5):677-84 PMID: 4320701
  7. Properties of incomplete Sendai virions and subgenomic viral RNAs.
    Virology. 1970 Dec;42(4):857-71 PMID: 4099084
  8. Electrophoretic distribution of the proteins and glycoproteins of influenza virus and Sendai virus.
    J Virol. 1970 Dec;6(6):707-16 PMID: 4322079
  9. Sendai virus replication: an ultrastructural comparison of productive and abortive infections in avian cells.
    J Gen Virol. 1970 Dec;9(3):169-77 PMID: 5532238
  10. Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus.
    J Virol. 1971 Jan;7(1):47-52 PMID: 5101090
  11. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase.
    J Virol. 1971 Mar;7(3):389-94 PMID: 4323712
  12. Ribonucleic acid polymerase activity in Sendai virions and nucleocapsid.
    J Virol. 1971 Jul;8(1):81-6 PMID: 4328418
  13. Ribonucleic acid transcriptases in Sendai Virions and infected cells.
    J Virol. 1971 Aug;8(2):174-80 PMID: 4329968
  14. Identification of transcriptive and replicative intermediates in Sendai virus-infected cells.
    Virology. 1972 Mar;47(3):711-25 PMID: 4335074
  15. RNA polymerase activity in virions of parainfluenza 1 virus (Sendai).
    Arch Gesamte Virusforsch. 1972;37(2):203-10 PMID: 4337544
  16. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  17. Amino acid metabolism in mammalian cell cultures.
    Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
  18. The isolation of a cell membrane fraction from rat liver.
    J Biophys Biochem Cytol. 1960 Oct;8:413-22 PMID: 13728607
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.5.1037-1043.1972
Published
1972-11-00
Pages
1037-43
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356575
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.5.1037-1043.1972 PMC Full Text View journal details
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