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PMID: 411876 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Suppression of human T-cell mitogenesis by prostaglandin. Existence of a prostaglandin-producing suppressor cell.

The Journal of experimental medicine ·Vol. 146 ·No. 6 ·1977-12-01 ·Pages 1719-34

Goodwin JS, Bankhurst AD, Messner RP

Abstract

Small amounts of PGE inhibit mitogen-induced [3H]thymidine incorporation in human peripheral lymphocytes. The 50% inhibitory concentration is approximately 10(-7) M, and this is reduced to approximately 10(-8) M when endogenous PGE production is blocked. PGE inhibits PHA- and Con A-stimulated cultures much better than PWM cultures, suggesting a differential effect of PGE on T-cell vs. B-cell function. In vitro blockade of PG synthesis results in approximately 50% increase in [3H]thymidine incorporation in PHA cultures. PGE is produced endogenously in PHA cultures by glass adherent suppressor cells.

MeSH Terms
Cyclooxygenase Inhibitors Lymphocyte Activation/drug effects Mitogens/pharmacology Monocytes/metabolism Prostaglandins E/pharmacology Thymidine/metabolism
Chemicals
Cyclooxygenase Inhibitors Mitogens Prostaglandins E Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goodwin J S
Bankhurst A D
Messner R P
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36 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.146.6.1719
Published
1977-12-01
Pages
1719-34
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2181914
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.146.6.1719 PMC Full Text View journal details
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