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PMID: 4119592 Published · ppublish English Journal Article

2,4-Dinitrophenyl receptors on mouse thymus and spleen cells.

The Journal of experimental medicine ·Vol. 137 ·No. 2 ·1973-02-01 ·Pages 470-82

Lawrence DA, Spiegelberg HL, Weigle WO

Abstract

A higher percentage of specific antigen-binding cells can be detected not only in normal CBA/J mouse spleen cell preparations (0.99%), but also in the normal thymus cell preparations (0.15%) with the use of [(125)I]2,4-dinitrophenyl-human IgG (DNP-HGG) as compared with most other antigens employed under similar conditions. The receptors on these cells are mainly specific for the DNP group as shown by the inhibition studies with DNP-lysine and the other DNP conjugates. In addition, it was shown by the inhibition studies with DNP-lysine that the thymus cells seem to have a lower avidity for DNP than the spleen cells. Preincubation of cell suspensions with antisera to immunoglobulins showed that the DNP-HGG antigen-binding cells in the thymus are inhibited predominantly with anti-micro-chain serum and the spleen cells with both anti-micro-chain and anti-gamma-chain sera; both cell populations were also significantly inhibited with the antisera to kappa-chains and Fab fragments. These data indicate that the nature of the receptor on the T cell differs from that on the majority of spleen cells.

MeSH Terms
Animals Antigens Autoradiography Binding Sites Cortisone/pharmacology Dinitrophenols Immune Sera Mice Mice, Inbred CBA Ovalbumin Serum Albumin, Bovine Spleen/immunology T-Lymphocytes/immunology gamma-Globulins
Chemicals
Antigens Dinitrophenols Immune Sera gamma-Globulins Serum Albumin, Bovine Ovalbumin Cortisone
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lawrence D A
Spiegelberg H L
Weigle W O
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38 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.137.2.470
Published
1973-02-01
Pages
470-82
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139479
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.137.2.470 PMC Full Text View journal details
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