Fractionation of the nucleus by divalent cations. Isolation of nuclear membranes.

The Journal of cell biology ·Vol. 55 ·No. 1 ·1972-10-00 ·Pages 104-25

Monneron A, Blobel G, Palade GE

Abstract

0.3-0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55-60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.

MeSH Terms

Animals Cations, Divalent Cell Fractionation Cell Nucleus/analysis Centrifugation DNA/analysis Electrophoresis, Polyacrylamide Gel Freeze Etching Liver Magnesium/pharmacology Membranes Microscopy, Electron Microsomes, Liver/analysis Nucleoproteins/analysis Peptides/analysis Potassium Chloride/pharmacology RNA/analysis Rats Sodium Dodecyl Sulfate Staining and Labeling

Chemicals

Cations, Divalent Nucleoproteins Peptides Sodium Dodecyl Sulfate RNA Potassium Chloride DNA Magnesium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Monneron A

Blobel G

Palade G E

References (19)

19 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.55.1.104

Published

1972-10-00

Pages

104-25

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2108744

Subset

External Links

PubMed source DOI: 10.1083/jcb.55.1.104 PMC Full Text View journal details

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Fractionation of the nucleus by divalent cations. Isolation of nuclear membranes.

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