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PMID: 4120095 Published · ppublish English Journal Article

Immunological tolerance to a hapten. I. Induction and maintenance of tolerance to trinitrophenyl with trinitrobenzene sulfonic acid.

The Journal of experimental medicine ·Vol. 137 ·No. 1 ·1973-01-01 ·Pages 42-54

Fidler JM, Golub ES

Abstract

Treatment of mice with a nonimmunogenic preparation of free reactive hapten, trinitrobenzene sulfonic acid (TNBS), leads to the induction of a state of tolerance to the hapten, 2,4,6-trinitrophenyl (TNP). This is determined by the lack of response to the haptenic moiety in an immunogenic hapten-carrier conjugate (TNP-SRBC) as assayed both by serum antibody titrations and the hemolytic plaque assay. The tolerance produced is specific for the hapten, since the anticarrier responses are essentially unaltered compared with the control values. The unresponsiveness induced by TNBS treatment is a dose-dependent phenomenon, becoming less complete at lower doses of TNBS. The tolerance is of a definite length, both in its induction phase and in the duration of the established unresponsive state. Tolerance can be maintained and extended, and may also be reentered once escape has been initiated.

MeSH Terms
Animals Antibody-Producing Cells Antigen-Antibody Reactions Drug Tolerance/drug effects Erythrocytes/immunology Hemolytic Plaque Technique Horses/immunology Immune Tolerance/drug effects Immunization Mice Mice, Inbred C57BL Mice, Inbred DBA Nitrobenzenes/administration & dosage,pharmacology Nitrophenols Sheep/immunology Sulfonic Acids Thymus Gland/immunology Time Factors gamma-Globulins
Chemicals
Nitrobenzenes Nitrophenols Sulfonic Acids gamma-Globulins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fidler J M
Golub E S
References (20)
20 references, click to expand
  1. Detection of cells producing 7S antibodies by the plaque technique.
    Nature. 1965 Nov 27;208(5013):858-9 PMID: 5870100
  2. Use of an antiglobulin serum to detect cells producing antibody with low haemolytic efficiency.
    Nature. 1965 Nov 27;208(5013):859-61 PMID: 4958328
  3. Mechanism of induction of immunological tolerance. II. Simultaneous development of priming and tolerance.
    Aust J Exp Biol Med Sci. 1966 Aug;44(4):327-40 PMID: 5967341
  4. Immunization of dissociated spleen cell cultures from normal mice.
    J Exp Med. 1967 Sep 1;126(3):423-42 PMID: 6034749
  5. Studies on the induction of immunologic unresponsiveness. II. Kinetics.
    J Immunol. 1967 Sep;99(3):624-8 PMID: 4168321
  6. A modification of the hemolytic plaque assay for use with protein antigens.
    J Immunol. 1968 Jan;100(1):133-7 PMID: 4170425
  7. Studies on the induction of immunologic unresponsiveness. 3. Antigen form and mouse strain variation.
    J Immunol. 1969 Feb;102(2):389-96 PMID: 4179469
  8. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen.
    Proc Soc Exp Biol Med. 1969 Nov;132(2):575-81 PMID: 5355110
  9. Contact and delayed hypersensitivity in the mouse. 3. Depression of contact sensitivity by pre-treatment with antigen and the restoration of immune competence in tolerant mice by normal lymphoid and bone marrow cells.
    Immunology. 1970 Jan;18(1):99-106 PMID: 4903986
  10. An in vitro primary immune response to 2,4,6-trinitrophenyl substituted erythrocytes: response against carrier and hapten.
    J Immunol. 1970 Jun;104(6):1558-61 PMID: 4911555
  11. Kinetic differences in unresponsiveness of thymus and bone marrow cells.
    Science. 1971 Feb 26;171(3973):813-5 PMID: 5099924
  12. Induction of immunological tolerance by a hapten (DNP) bound to a non-immunogenic protein carrier.
    Nat New Biol. 1971 Apr 7;230(14):180-2 PMID: 4928923
  13. Induction of immunity and tolerance to the dinitrophenyl determinant in vitro.
    Nat New Biol. 1971 May 5;231(18):21-3 PMID: 4103761
  14. Infectious immunological tolerance.
    Immunology. 1971 Dec;21(6):903-14 PMID: 4943147
  15. Human serum macroglobulins and dissociation units. I. Physicochemical properties.
    J Biol Chem. 1958 Apr;231(2):1107-18 PMID: 13539040
  16. Specific inhibition of antibody production. II. Paralysis induced in adult mice by small quantities of protein antigen.
    Immunology. 1962 May;5:378-88 PMID: 13887798
  17. Unusual sedimentation and sulfhydryl sensitivity of certain isohemagglutinins and skin-sensitizing antibody.
    Proc Soc Exp Biol Med. 1962 May;110:101-5 PMID: 14493062
  18. TYPE OF ANTIBODY ELEVATED BY 5-FLUORO-2-DEOXYURIDINE AND ENDOTOXIN DURING THE PRIMARY RESPONSE OF THE MOUSE.
    Proc Soc Exp Biol Med. 1964 Apr;115:1132-6 PMID: 14166578
  19. INHIBITION OF 19S ANTIBODY SYNTHESIS BY 7S ANTIBODY.
    Science. 1964 Jul 24;145(3630):395-7 PMID: 14172600
  20. Tolerance of erythrocytes in poultry:induction and specificity.
    Immunology. 1962 May;5:341-58 PMID: 14474652
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.137.1.42
Published
1973-01-01
Pages
42-54
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139365
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.137.1.42 PMC Full Text View journal details
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