Physiological studies of methane- and methanol-oxidizing bacteria: immunological comparison of a primary alcohol dehydrogenase from Methylococcus capsulatus and Pseudomonas sp. M27.

Journal of bacteriology ·Vol. 113 ·No. 2 ·1973-02-00 ·Pages 937-45

Patel RN, Mandy WJ, Hoare DS

Abstract

A primary alcohol dehydrogenase was purified from cell extracts of two apparently unrelated microorganisms, namely, Pseudomonas sp. M27 and Methylococcus capsulatus. Rabbit antiserum prepared against the purified enzyme from M. capsulatus revealed distinctive antigenic determinants by quantitative and gel precipitin reactions. Rabbit antiserum to M27 enzyme detected both distinctive and shared antigenic determinants. Certain methane- and methanol-oxidizing bacteria were grouped on the basis of serological cross-reacting enzyme specificities.

MeSH Terms

Alcohol Oxidoreductases/analysis,antagonists & inhibitors,isolation & purification,metabolism Animals Bacteria/enzymology,immunology,metabolism Cell-Free System Cross Reactions Electrophoresis, Polyacrylamide Gel Epitopes Immune Sera Immunodiffusion Immunoelectrophoresis Immunoglobulin G/pharmacology Methane/metabolism Methanol/metabolism Precipitin Tests Pseudomonas/enzymology,immunology,metabolism Rabbits/immunology Species Specificity Spectrophotometry Ultracentrifugation

Chemicals

Epitopes Immune Sera Immunoglobulin G Alcohol Oxidoreductases Methane Methanol

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Patel R N

Mandy W J

Hoare D S

References (18)

18 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.113.2.937-945.1973

Published

1973-02-00

Pages

937-45

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285312

Subset

External Links

PubMed source DOI: 10.1128/jb.113.2.937-945.1973 PMC Full Text View journal details

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Physiological studies of methane- and methanol-oxidizing bacteria: immunological comparison of a primary alcohol dehydrogenase from Methylococcus capsulatus and Pseudomonas sp. M27.

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