Home LiteratureArticle Details
PMID: 4120861 Published · ppublish English Journal Article

Some properties of embryonic myosin.

The Journal of cell biology ·Vol. 55 ·No. 3 ·1972-12-00 ·Pages 586-94

Sreter F, Holtzer S, Gergely J, Holtzer H

Abstract

Myosins from the following sources were purified by diethylaminoethyl-Sephadex chromatography: moytubes grown in vitro for 7-8 days, prepared from pectoralis muscles of 10-day old embryos, and breast and leg muscles from 16-day old embryos. The adenosine triphosphatase activities of these myosins were close to that of adult m. pectoralis myosin. The light chains of the embryonic myosins had the same mobilities in sodium dodecyl sulfate electrophoresis as those in adult pectoralis muscle myosin and were clearly distinguishable from those in myosin from tonic muscle m. latissimus dorsi anterior. The fastest light chain in embryonic muscle myosin-apparent mol wt 16,000-was present in smaller amounts than in adult myosin. The negative staining pattern of paracrystals of embryonic light meromyosin (LMM) was indistinguishable from that of adult fast muscle LMM. The significance of these results for differentiation of various muscle types has been discussed.

MeSH Terms
Adenosine Triphosphatases/analysis Animals Chick Embryo Chromatography, Ion Exchange Culture Techniques Electrophoresis, Polyacrylamide Gel Macromolecular Substances Microscopy, Electron Muscle Development Muscles/embryology,enzymology Myosin Subfragments Myosins/analysis,isolation & purification Sodium Dodecyl Sulfate Staining and Labeling
Chemicals
Macromolecular Substances Myosin Subfragments Sodium Dodecyl Sulfate Adenosine Triphosphatases Myosins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sreter F
Holtzer S
Gergely J
Holtzer H
References (19)
19 references, click to expand
  1. An analysis of myogenesis in vitro using fluorescein-labeled antimyosin.
    J Histochem Cytochem. 1965 Nov-Dec;13(8):726-39 PMID: 5325593
  2. Ultrastructural aspects of myogenesis in the chick.
    Lab Invest. 1966 May;15(5):836-63 PMID: 5329986
  3. Studies on myosin from red and white skeletal muscles of the rabbit. I. Adenosine triphosphatase activity.
    J Biol Chem. 1966 Dec 25;241(24):5772-6 PMID: 4224728
  4. 3-Methyl histidine and adult and foetal forms of skeletal muscle myosin.
    Nature. 1968 Feb 3;217(5127):452-3 PMID: 4966706
  5. The adenosine triphosphatase and calcium ion-transporting activities of the sarcoplasmic reticulum of developing musce.
    Biochem J. 1969 Sep;114(2):161-70 PMID: 4241474
  6. Postnatal differentiation of the enzyme activity pattern of energy-supplying metabolism in slow (red) and fast (white) muscles of chicken.
    Eur J Biochem. 1970 Apr;13(2):289-92 PMID: 5439933
  7. The absence of 3-methylhistidine in red, cardiac and fetal myosins.
    Biochem Biophys Res Commun. 1970 Jun 5;39(5):956-64 PMID: 5423833
  8. Postnatal development of fiber types in normal and dystrophic skeletal muscle of the chick.
    Exp Neurol. 1971 Mar;30(3):431-46 PMID: 4251991
  9. Studies on muscle differentiation. 3. Immuno-histochemical identification of tropomyosin in skeletal and cardiac muscles of developing chick embryo.
    Dev Growth Differ. 1971 Feb;12(4):241-52 PMID: 4929012
  10. Light chains of myosins from white, red, and cardiac muscles.
    Proc Natl Acad Sci U S A. 1971 May;68(5):946-50 PMID: 4252540
  11. Changes in the properties of myosin associated with muscle development.
    Biochemistry. 1971 Apr 13;10(8):1316-21 PMID: 4253008
  12. Comparative studies of light meromyosin paracrystals derived from red, white, and cardiac muscle myosins.
    J Cell Biol. 1971 Jun;49(3):883-98 PMID: 4103957
  13. Electrophoretic studies of muscle proteins. II. Complex formation between delta protein, myogen and myosin.
    J Cell Physiol. 1971 Jun;77(3):281-300 PMID: 5104784
  14. Identification of the essential light chains of myosin.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1107-10 PMID: 4256613
  15. The synthesis and assembly of myofibrils in embryonic muscle.
    Curr Top Dev Biol. 1970;5:235-80 PMID: 4949637
  16. An analysis of myogenesis by the use of fluorescent antimyosin.
    J Biophys Biochem Cytol. 1957 Sep 25;3(5):705-24 PMID: 13475387
  17. ULTRASTRUCTURE OF DEVELOPING MUSCLE CELLS IN THE CHICK EMBRYO.
    Am J Anat. 1965 Jan;116:115-47 PMID: 14283278
  18. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
  19. Formation of arrowhead complexes with heavy meromyosin in a variety of cell types.
    J Cell Biol. 1969 Nov;43(2):312-28 PMID: 5344150
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.55.3.586
Published
1972-12-00
Pages
586-94
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108824
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.55.3.586 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2