Home LiteratureArticle Details
PMID: 4121027 Published · ppublish English Journal Article

DNA polymerase activities of human milk.

Science (New York, N.Y.) ·Vol. 180 ·No. 4082 ·1973-04-15 ·Pages 198-201

Gerwin BI, Ebert PS, Chopra HC, Smith SG, Kvedar JP, Albert S, Brennan MJ

Abstract

DNA polymerases have been partially purified from human milk. A DNA polymerase detected by phosphocellulose chromatography is similar to the enzymes of RNA tumor viruses in that a hybrid of polyriboadenylate and oligodeoxythymidylate is a better template than is DNA. However, this polymerase differed from that of the RNA tumor viruses in its chromatographic behavior. Three different methods of detecting "reverse transcriptase" activity failed to correlate with the donor's family history of cancer.

MeSH Terms
Breast Neoplasms/enzymology Centrifugation, Density Gradient Chromatography DNA Nucleotidyltransferases/isolation & purification,metabolism Female Humans Methods Milk, Human/enzymology Oncogenic Viruses/enzymology RNA Viruses/enzymology RNA-Directed DNA Polymerase/isolation & purification Thymine Nucleotides/metabolism Tritium
Chemicals
Thymine Nucleotides Tritium DNA Nucleotidyltransferases RNA-Directed DNA Polymerase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Gerwin B I
Ebert P S
Chopra H C
Smith S G
Kvedar J P
Albert S
Brennan M J
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
Published
1973-04-15
Pages
198-201
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]