Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls.

Infection and immunity ·Vol. 7 ·No. 3 ·1973-03-00 ·Pages 487-92

Gerber H, Horisberger M, Bauer H

Abstract

A procedure is given for the insolubilization of yeast mannan by entrapment in a polyacrylamide gel. Specific anti-mannan antibodies were separated by immunosorption from all other antibodies of a rabbit antiserum against whole, Formalininactivated Candida utilis cells. Colloidal gold coated with the anti-mannan and anti-non-mannan antibody fractions was used for the ultrastructural visualization of cell wall antigens. A control was performed using normal rabbit serum. Inhibition of anti-mannan antibodies was obtained with mannan oligosaccharides, and none of the antibody fractions showed marking of Saccharomyces cerevisiae cell walls.

MeSH Terms

Animals Antibodies, Fungal Antibody Specificity Antigens, Fungal Candida/immunology Cell Wall Epitopes Immune Sera Rabbits/immunology Saccharomyces cerevisiae/immunology

Chemicals

Antibodies, Fungal Antigens, Fungal Epitopes Immune Sera

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gerber H

Horisberger M

Bauer H

References (12)

12 references, click to expand

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Preparation of polyacrylamide gels which contain protein and their use as high capacity immunosorbents.
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Mercury-labelled concanavalin A as a marker in electron microscopy-localisation of mannan in yeast cell walls.
FEBS Lett. 1971 Nov 1;18(2):311-314 PMID: 11946150
Method for fingerprinting yeast cell wall mannans.
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Mannan as a major component of the bud scars of Saccharomyces cerevisiae.
Arch Mikrobiol. 1972;85(3):202-8 PMID: 4562154
ANTIGENS AND ENZYMES MADE INSOLUBLE BY ENTRAPPING THEM INTO LATTICES OF SYNTHETIC POLYMERS.
Science. 1963 Nov 8;142(3593):678-9 PMID: 14068215
An immunocolloid method for the electron microscope.
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A study of the immunochemistry of three yeast mannans.
J Biol Chem. 1970 Mar 10;245(5):1197-203 PMID: 5461381
Entrapment of collagen in a polyacrylamide matrix and its application in the purification of animal collagenases.
Biochim Biophys Acta. 1972 May 18;263(3):564-73 PMID: 4338310
Comparison of the effects of various detergents on antigen-antibody interaction.
FEBS Lett. 1972 May 1;22(2):210-212 PMID: 11946599
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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.7.3.487-492.1973

Published

1973-03-00

Pages

487-92

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC422705

Subset

External Links

PubMed source DOI: 10.1128/iai.7.3.487-492.1973 PMC Full Text View journal details

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Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls.

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