In vitro production of mouse mammary tumor virus in a mouse mammary tumor ascites line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 10 ·1973-10-00 ·Pages 2983-7

Keydar J, Gilead Z, Hartman JR, Ben-Shaul Y

Abstract

An ascites line derived from a spontaneous mouse mammary carcinoma produces, on explantation and cultivation in vitro, large amounts of oncornavirus particles. The biochemical, biophysical, and electron microscopic characteristics of the virions are described. Molecular hybridization and immunological methods identify these virions as mouse mammary tumor virus.

MeSH Terms

Animals Antigens, Viral/analysis Ascitic Fluid Cell Line Centrifugation, Density Gradient Complement Fixation Tests DNA, Viral/analysis,biosynthesis Mammary Neoplasms, Experimental/microbiology Mammary Tumor Virus, Mouse/immunology,isolation & purification Mice Microscopy, Electron Neoplasm Transplantation Nucleic Acid Hybridization Polynucleotides RNA RNA, Viral/analysis RNA-Directed DNA Polymerase/metabolism Templates, Genetic

Chemicals

Antigens, Viral DNA, Viral Polynucleotides RNA, Viral RNA RNA-Directed DNA Polymerase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Keydar J

Gilead Z

Hartman J R

Ben-Shaul Y

References (27)

27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.10.2983

Published

1973-10-00

Pages

2983-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427152

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.10.2983 PMC Full Text View journal details

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In vitro production of mouse mammary tumor virus in a mouse mammary tumor ascites line.

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