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PMID: 4126768 Published · ppublish English Journal Article

Thymus-derived rosettes are not "helper" cells.

The Journal of experimental medicine ·Vol. 138 ·No. 5 ·1973-11-01 ·Pages 1133-43

Elliott BE, Haskill JS, Axelrad MA

Abstract

Rosettes against SRBC were made from normal spleen cells. Although T rosettes tend to dissociate, they could be stabilized with 0.05% sodium azide. A clear separation of nonrosettes, T rosettes, and B rosettes was obtained by subjecting the suspension of splenic rosettes to velocity sedimentation at unit gravity. Each fraction was injected with either normal bone marrow cells or normal thymus cells with antigen into 650-R-irradiated hosts. Direct plaque-forming cells (PFC) were assayed in the spleens 7 days later. Synergism with thymus cells occurred only in the B-rosette fraction; PFC precursors therefore sedimented as B rosettes. Synergism with bone marrow cells occurred only in the nonrosette small lymphocyte fraction; helper cells therefore did not bind detectable numbers of sheep red blood cells (SRBC). Thus T rosettes are not helper cells in the direct PFC response of bone marrow B cells to SRBC.

MeSH Terms
Animals Antibody-Producing Cells B-Lymphocytes/immunology Binding Sites, Antibody Epitopes Erythrocytes/immunology Immune Adherence Reaction Sheep/immunology Spleen/immunology T-Lymphocytes/immunology
Chemicals
Epitopes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Elliott B E
Haskill J S
Axelrad M A
References (34)
34 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.138.5.1133
Published
1973-11-01
Pages
1133-43
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139438
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.138.5.1133 PMC Full Text View journal details
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