Crystal formation by a ribonucleic acid polymerase mutant of Bacillus subtilis.

Journal of bacteriology ·Vol. 116 ·No. 1 ·1973-10-00 ·Pages 479-82

Santo LY, Doi RH

Abstract

A crystalline inclusion has been observed in a ribonucleic acid polymerase mutant of Bacillus subtilis which is conditionally temperature sensitive only during sporulation. The crystal is formed at the permissive temperature in 1 to 2% of the sporulating (stage III-IV) cells; about 85% of the cells sporulate normally, while the cells with crystals do not sporulate. The wild type does not form crystals at either the permissive (30 C) or the nonpermissive (47 C) temperatures. The crystal may result from altered transcription during sporulation at 30 C.

MeSH Terms

Bacillus subtilis/cytology,enzymology,growth & development Crystallography DNA-Directed RNA Polymerases/biosynthesis Inclusion Bodies Microscopy, Electron Mutation Spores, Bacterial/growth & development Staining and Labeling Temperature Transcription, Genetic

Chemicals

DNA-Directed RNA Polymerases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Santo L Y

Doi R H

References (7)

7 references, click to expand

Studies on the structure of parasporal inclusions from Bacillus thuringiensis.
J Mol Biol. 1965 Dec;14(2):572-81 PMID: 5880865
Biochemical homology between crystal and spore protein of Bacillus thuringiensis.
J Bacteriol. 1968 Sep;96(3):721-6 PMID: 5732505
Bacillus subtilis mutant altered in spore morphology and in RNA polymerase activity.
Proc Natl Acad Sci U S A. 1970 Jun;66(2):404-10 PMID: 4988921
Electron microscopy of the altered spore morphology of a ribonucleic acid polymerase mutant of Bacillus subtilis.
J Bacteriol. 1971 Mar;105(3):1110-8 PMID: 4994032
An RNA polymerase mutation causing temperature-sensitive sporulation in Bacillus subtilis.
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1179-83 PMID: 4197929
Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states.
J Biophys Biochem Cytol. 1958 Nov 25;4(6):671-8 PMID: 13610928
The protein crystals of Bacillus thuringiensis Berliner.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.116.1.479-482.1973

Published

1973-10-00

Pages

479-82

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC246445

Subset

External Links

PubMed source DOI: 10.1128/jb.116.1.479-482.1973 PMC Full Text View journal details

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Crystal formation by a ribonucleic acid polymerase mutant of Bacillus subtilis.

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