Transcription of DNA from the 70S RNA of Rous sarcoma virus. I. Identification of a specific 4S RNA which serves as primer.

Journal of virology ·Vol. 13 ·No. 5 ·1974-05-00 ·Pages 1126-33

Dahlberg JE, Sawyer RC, Taylor JM, Faras AJ, Levinson WE, Goodman HM, Bishop JM

Abstract

The enzymatic transcription of DNA from the 70S RNA of Rous sarcoma virus (RSV) is initiated on the 3' terminus of a molecule of 4S RNA which is hydrogen bonded to the viral genome. We labeled this primer with radioactive deoxynucleotides, and demonstrated that its release from 70S RNA by thermal denaturation was accompanied by a reduction in the template activity of the viral RNA. Two-dimensional electrophoresis in polyacrylamide gels separated the 4S RNAs associated with the 70S RNA of RSV into approximately eight fractions, each of which appeared to contain a discrete species of tRNA. The RNA in one of these fractions served as the principal primer for initiation of DNA synthesis by both detergent-disrupted virions of RSV and purified RNA-directed DNA polymerase with RSV 70S RNA as template.

MeSH Terms

Avian Sarcoma Viruses/enzymology DNA/biosynthesis Electrophoresis, Polyacrylamide Gel Hot Temperature Molecular Weight Nucleic Acid Denaturation Phosphorus Radioisotopes RNA, Viral RNA-Directed DNA Polymerase/metabolism Templates, Genetic Transcription, Genetic

Chemicals

Phosphorus Radioisotopes RNA, Viral DNA RNA-Directed DNA Polymerase

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Dahlberg J E

Sawyer R C

Taylor J M

Faras A J

Levinson W E

Goodman H M

Bishop J M

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30 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.13.5.1126-1133.1974

Published

1974-05-00

Pages

1126-33

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355423

Subset

External Links

PubMed source DOI: 10.1128/JVI.13.5.1126-1133.1974 PMC Full Text View journal details

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Transcription of DNA from the 70S RNA of Rous sarcoma virus. I. Identification of a specific 4S RNA which serves as primer.

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