Immunohistochemical demonstration of IgG in Reed-Sternberg and other cells in Hodgkin's disease.

The Journal of experimental medicine ·Vol. 139 ·No. 5 ·1974-05-01 ·Pages 1077-83

Garvin AJ, Spicer SS, Parmley RT, Munster AM

Abstract

Spleens and lymph nodes fixed lightly for optimal immunocytochemistry or processed routinely for surgical diagnosis disclosed strong selective immunostaining for IgG in numerous immunocytes in tumor-free areas. Areas involved by Hodgkin's disease revealed, in addition, strong immunostaining for IgG but not IgM in Reed-Sternberg cells and faint to strong staining in Hodgkin cells as well. Ultrastructurally the Reed-Sternberg and Hodgkin cells displayed abundant polyribosomes and sparse granular reticulum and appeared to form unexportable IgG on unbound ribosomes.

MeSH Terms

Antibody-Producing Cells/immunology Histocytochemistry Hodgkin Disease/immunology Humans Immunochemistry Immunoglobulin G/analysis Lymph Nodes/cytology,immunology Microscopy, Electron Peroxidases Spleen/cytology,immunology Staining and Labeling

Chemicals

Immunoglobulin G Peroxidases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Garvin A J

Spicer S S

Parmley R T

Munster A M

References (5)

5 references, click to expand

An immunoglobulin-enzyme bridge method for localizing tissue antigens.
J Histochem Cytochem. 1969 Sep;17(9):563-9 PMID: 4186250
"Reed-Sternberg cells" in infectious mononucleosis?
Am J Clin Pathol. 1972 Dec;58(6):607-17 PMID: 4118369
Immunoglobulin-positive Reed Sternberg cells in Hodgkin's disease.
Lancet. 1973 Aug 4;2(7823):265-6 PMID: 4124458
In vitro splenic IgG synthesis in Hodgkin's disease.
N Engl J Med. 1973 Oct 11;289(15):763-7 PMID: 4542304
Differentiation of nucleic acids by staining at controlled pH and by a Schiff-methylene blue sequence.
Stain Technol. 1961 Nov;36:337-40 PMID: 13915747

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.139.5.1077

Published

1974-05-01

Pages

1077-83

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2139657

Subset

External Links

PubMed source DOI: 10.1084/jem.139.5.1077 PMC Full Text View journal details

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Immunohistochemical demonstration of IgG in Reed-Sternberg and other cells in Hodgkin's disease.

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