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PMID: 4138694 Published · ppublish English Journal Article

Macrophage-lymphocyte clusters in the immune response to soluble protein antigen in vitro. II. Ultrastructure of clusters formed during the early response.

The Journal of experimental medicine ·Vol. 140 ·No. 5 ·1974-11-01 ·Pages 1260-72

Nielsen MH, Jensen H, Braendstrup O, Werdelin O

Abstract

Macrophage-lymphocyte clusters are formed when lymph node cells and autologous peritoneal exudate cells from guinea pigs immunized with tubercle bacilli are cultured in the presence of purified protein derivative of tuberculin (PPD) for 20 h. We have studied the ultrastructure of these clusters employing transmission and scanning electron microscopy. The most simple macrophage-lymphocyte cluster consisted of one macrophage, one large central lymphocyte with a blastoid appearance attached to the macrophage with a broad area of contact, and from a few to more than 20 small peripheral lymphocytes attached to the central lymphocyte by their uropods. Some clusters were of more complex type, containing two or three macrophages or one macrophage with more than one central lymphocyte attached to the surface, but even in these clusters each peripheral lymphocyte was attached only to one central lymphocyte. By morphological criteria the peripheral lymphocytes were T lymphocytes.

MeSH Terms
Animals Binding Sites, Antibody Cell Membrane/ultrastructure Cells, Cultured Culture Media Epitopes Female Guinea Pigs Immunity, Cellular Immunization In Vitro Techniques Lymph Nodes/cytology Lymphocytes/immunology,ultrastructure Macrophages/immunology,ultrastructure Male Microscopy, Electron Microscopy, Electron, Scanning Peritoneum/cytology T-Lymphocytes/immunology Tuberculin
Chemicals
Culture Media Epitopes Tuberculin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nielsen M H
Jensen H
Braendstrup O
Werdelin O
References (40)
40 references, click to expand
  1. Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters.
    J Exp Med. 1969 Feb 1;129(2):351-62 PMID: 5762048
  2. Autologous macrophage-thymocyte interactions.
    J Allergy. 1970 Sep;46(3):190-4 PMID: 4918208
  3. Macrophage-lymphocyte clusters in the immune response to soluble protein antigen in vitro.
    J Exp Med. 1974 Nov 1;140(5):1245-59 PMID: 4138693
  4. Microspikes on the lymphocyte uropod.
    Science. 1969 Feb 21;163(3869):818-20 PMID: 5764475
  5. IMMUNOGENICITY OF ANTIGEN-CONTAINING RIBONUCLEIC ACID PREPARATIONS FROM MACROPHAGES.
    Nature. 1965 Jan 30;205:470-4 PMID: 14269699
  6. Mediation of delayed hypersensitivity by peripheral blood lymphocytes in vitro and by their products in vivo and in vitro, morphology of in vitro lymphocyte-macrophage interaction.
    J Immunol. 1968 May;100(5):1000-5 PMID: 5648472
  7. Identification of human B and T lymphocytes by scanning electron microscopy.
    J Exp Med. 1973 Sep 1;138(3):607-24 PMID: 4542254
  8. Selective triggering of human T and B lymphocytes in vitro by polyclonal mitogens.
    J Exp Med. 1974 Jul 1;140(1):1-18 PMID: 4600344
  9. Ultrastructural analysis of hemolysin-forming cell clusters. I. Preliminary observations.
    J Immunol. 1970 Nov;105(5):1299-303 PMID: 5531451
  10. Communications. "In vitro" cell reactions in delayed hypersensitivity.
    J Immunol. 1969 Jul;103(1):138-41 PMID: 4978776
  11. Cellular interactions and the secondary response in vitro.
    Nat New Biol. 1971 Feb 24;229(8):254-5 PMID: 5279801
  12. Antibody formation in vitro.
    J Exp Med. 1961 Dec 1;114:837-56 PMID: 13893304
  13. Macrophage-lymphocyte interaction in the antigen-induced blastogenic response of human peripheral blood leukocytes.
    J Immunol. 1968 Jun;100(6):1184-94 PMID: 5696282
  14. Morphological observations on the cytotoxicity of human bood lymphocytes for antibody-coated chicken erythrocytes.
    Exp Cell Res. 1970 Oct;62(2):433-40 PMID: 5531378
  15. Association between macrophages and specific antibody producing cells.
    Nat New Biol. 1971 Feb 10;229(6):184-5 PMID: 5280108
  16. Synergistic interaction of macrophages and lymphocytes in antigen-induced transformation of lymphocytes.
    J Exp Med. 1970 Jul 1;132(1):44-65 PMID: 4994445
  17. Activity in vitro of lymphocytes and macrophages in delayed hypersensitivity.
    J Immunol. 1971 Sep;107(3):655-62 PMID: 4999089
  18. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
    J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
  19. Antigen-induced proliferation of guinea pig lymphocytes in vitro: obligatory role of macrophages in the recognition of antigen by immune T-lymphocytes.
    J Immunol. 1973 Jul;111(1):58-64 PMID: 4541326
  20. Studies on macrophage RNA involved in antibody production.
    Proc Natl Acad Sci U S A. 1967 Jun;57(6):1849-56 PMID: 5231416
  21. Functional anatomy of the lymphocyte in immunological reactions in vitro.
    J Exp Med. 1966 Nov 1;124(5):851-8 PMID: 5926299
  22. Cellular interactions in the proliferative response of human T and B lymphocytes to phytomitogens and allogeneic lymphocytes.
    J Exp Med. 1974 Jun 1;139(6):1553-67 PMID: 4598019
  23. Macrophage-lymphocyte interaction. I. Characteristics of the antigen-independent-binding of guinea pig thymocytes and lymphocytes to syngeneic macrophages.
    J Exp Med. 1973 Oct 1;138(4):900-24 PMID: 4744011
  24. The uropod-bearing lymphocyte of the guinea pig. Evidence for thymic origin.
    J Exp Med. 1972 May 1;135(5):1037-48 PMID: 4623313
  25. The interaction of human monocytes and lymphocytes.
    J Exp Med. 1968 Dec 1;128(6):1309-25 PMID: 5688078
  26. Function of macrophages in antigen recognition by guinea pig T lymphocytes. II. Role of the macrophage in the regulation of genetic control of the immune response.
    J Exp Med. 1973 Nov 1;138(5):1213-29 PMID: 4126770
  27. Bridge formation between mouse peritoneal macrophage-macrophage and macrophage-lymph node cells and the influence of various chemicals.
    J Reticuloendothel Soc. 1972 Jun;11(6):579-98 PMID: 4558261
  28. The lymphocyte in immunological reactions in vitro: ultrastructural studies.
    Blood. 1970 May;35(5):683-8 PMID: 4910964
  29. A ROUTINE TECHNIQUE FOR DOUBLE-STAINING ULTRATHIN SECTIONS USING URANYL AND LEAD SALTS.
    J Cell Biol. 1965 Apr;25:157-61 PMID: 14289358
  30. CYTOPLASMIC INTERACTION BETWEEN MACROPHAGES AND LYMPHOCYTIC CELLS IN ANTIBODY SYNTHESIS.
    Science. 1964 Feb 28;143(3609):964-5 PMID: 14090148
  31. An ultrastructural study of lymphocytes with surface-bound immunoglobulin.
    J Exp Med. 1972 Feb 1;135(2):267-76 PMID: 4536751
  32. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes.
    J Exp Med. 1973 Nov 1;138(5):1194-212 PMID: 4542806
  33. Electron microscopic study of the lymphocytes capable of binding antigen-antibody-complement complexes.
    Cell Immunol. 1972 Jul;4(3):279-88 PMID: 5037347
  34. Uropod formation in phytohaemagglutinin (PHA) stimulated lymphocytes.
    Exp Cell Res. 1971 Jun;66(2):433-45 PMID: 4326615
  35. In vitro transfer of macrophage RNA to lymph node cells.
    Nature. 1963 May 11;198:549-51 PMID: 13945312
  36. Mouse thymus-independent and thymus-derived lymphoid cells. II. Ultrastructural studies.
    J Exp Med. 1972 Nov 1;136(5):1008-30 PMID: 4563148
  37. LYMPHOCYTE FOOT APPENDAGE: ITS ROLE IN LYMPHOCYTE FUNCTION AND IN IMMUNOLOGICAL REACTIONS.
    Nature. 1965 Feb 27;205:887-8 PMID: 14293154
  38. Tolerance, enhancement and the regulation of interactions between T cells, B cells and macrophages.
    Transplant Rev. 1972;13:3-34 PMID: 4142145
  39. Human monocytes and macrophages. Interaction with antigen and lymphocytes.
    J Cell Biol. 1970 Jul;46(1):97-105 PMID: 5459014
  40. Antibody formation initiated in vitro. II. Antibody synthesis in x-irradiated recipients of diffusion chambers containing nucleic acid derived from macrophages incubated with antigen.
    J Exp Med. 1963 Apr 1;117:595-602 PMID: 13945311
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.140.5.1260
Published
1974-11-01
Pages
1260-72
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139730
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.140.5.1260 PMC Full Text View journal details
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