Protective and cellular immune responses to idiotypic determinants on cells from a spontaneous lymphoma of NZB-NZW F1 mice.

The Journal of experimental medicine ·Vol. 140 ·No. 6 ·1974-12-01 ·Pages 1547-58

Sugai S, Palmer DW, Talal N, Witz IP

Abstract

A spontaneous lymphoma (141) producing monoclonal IgM is established in NZB/NZW F(1) (B/W) mice who spontaneously develop an autoimmune disease. Idiotypic determinants of 141 IgM are present on the lymphoma cell surface as shown by indirect immunofluorescence and specific cytotoxicity with rabbit anti-idiotypic antiserum. Fluorescence and cytotoxicity are inhibited by 141 IgM but not by 104E IgM, a monoclonal IgM produced by a BALB/c plasmacytoma. Immunization of B/W mice with 141 IgM before transplantation of lymphoma 141 confers protective immunity. No such protection occurs after immunization with 104E IgM or other unrelated proteins. Protected mice contain spleen cells cytotoxic for 141 lymphoma cells. This cytotoxicity is blocked by incubation of spleen cells with 141 IgM but not with 104E IgM. Moreover, splenic lymphocytes from protected mice are stimulated to synthesize DNA by 141 IgM but not by 104E IgM. These results suggest that specific cellular immune responses to idiotypic determinants may participate in the observed protection against challenge with the corresponding B-cell tumor.

MeSH Terms

Animals Antibodies, Anti-Idiotypic Antibody Specificity B-Lymphocytes/immunology Cell Membrane/immunology Chromium Radioisotopes Cytotoxicity Tests, Immunologic Epitopes Fluorescent Antibody Technique Immunity, Cellular Immunization Immunoglobulin M Lymphocyte Activation Lymphocytes/immunology,ultrastructure Lymphoma/immunology,prevention & control Mice Mice, Inbred NZB Plasmacytoma/immunology Thymidine/metabolism Tritium

Chemicals

Antibodies, Anti-Idiotypic Chromium Radioisotopes Epitopes Immunoglobulin M Tritium Thymidine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Sugai S

Palmer D W

Talal N

Witz I P

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26 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.140.6.1547

Published

1974-12-01

Pages

1547-58

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2139761

Subset

External Links

PubMed source DOI: 10.1084/jem.140.6.1547 PMC Full Text View journal details

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Protective and cellular immune responses to idiotypic determinants on cells from a spontaneous lymphoma of NZB-NZW F1 mice.

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