The effects of streptomycin or dihydrostreptomycin binding to 16S RNA or to 30S ribosomal subunits.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 10 ·1974-10-00 ·Pages 3814-8

Garvin RT, Biswas DK, Gorini L

Abstract

Evidence is presented suggesting that streptomycin binds to 16S RNA or to 30S ribosomal subunits at the same topographical site located on the RNA chain. The equally bactericidal dihydrostreptomycin binds to the same site as streptomycin but with lower affinity. The effect of drug binding to 16S RNA (measured by reconstitution inhibition) is readily reversible, while that of drug binding to 30S subunits (measured by misreading) persists after removal of the drug. Binding of the drug is not a necessary and sufficient reason for killing.

MeSH Terms

Carbon Radioisotopes Cell Fractionation Dihydrostreptomycin Sulfate/metabolism Escherichia coli/metabolism,ultrastructure Genotype Phenotype RNA, Bacterial/metabolism RNA, Ribosomal/metabolism Ribosomes/metabolism Streptomycin/metabolism Tritium

Chemicals

Carbon Radioisotopes RNA, Bacterial RNA, Ribosomal Tritium Dihydrostreptomycin Sulfate Streptomycin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Garvin R T

Biswas D K

Gorini L

References (15)

15 references, click to expand

Phenotype masking and streptomycin dependence.
Science. 1967 Sep 15;157(3794):1314-7 PMID: 5341602
Binding of dihydrostreptomycin to ribosomal subunits.
J Mol Biol. 1968 Mar 14;32(2):221-30 PMID: 4171195
Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli.
Nature. 1969 Apr 26;222(5191):333-9 PMID: 4181187
Genetic analysis of streptomycin resistance in Escherichia coli.
Genetics. 1970 May;65(1):9-25 PMID: 4920897
Thin layer chromatography (bioautography) of streptomycins.
Acta Chem Scand. 1970;24(8):3086-7 PMID: 5507494
Nature of phenotypic masking exhibited by drug-dependent streptomycin A mutants of Escherichia coli.
J Mol Biol. 1971 May 14;57(3):403-22 PMID: 4931676
A ribosomal ambiguity mutation.
J Mol Biol. 1969 Jan 14;39(1):95-112 PMID: 4938819
Alteration of a 30S ribosomal protein accompanying the ram mutation in Escherichia coli.
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2263-7 PMID: 4943794
The attachment site of streptomycin to the 30S ribosomal subunit.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2141-4 PMID: 4559597
Ribosomal assembly influenced by growth in the presence of streptomycin.
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2762-6 PMID: 4583024
Protein involved in the binding of dihydrostreptomycin to ribosomes of Escherichia coli.
J Mol Biol. 1973 Nov 25;81(1):71-82 PMID: 4129206
Binding of dihydrostreptomycin to Escherichia coli ribosomes: characteristics and equilibrium of the reaction.
Antimicrob Agents Chemother. 1972 Oct;2(4):294-307 PMID: 4133236
PHENOTYPIC REPAIR BY STREPTOMYCIN OF DEFECTIVE GENOTYPES IN E. COLI.
Proc Natl Acad Sci U S A. 1964 Mar;51:487-93 PMID: 14171463
Transduction of linked genetic characters of the host by bacteriophage P1.
Virology. 1955 Jul;1(2):190-206 PMID: 13267987
Isolation and characterization of ribonuclease I mutants of Escherichia coli.
J Mol Biol. 1966 Mar;16(1):67-84 PMID: 5331244

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.10.3814

Published

1974-10-00

Pages

3814-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC434274

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.10.3814 PMC Full Text View journal details

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The effects of streptomycin or dihydrostreptomycin binding to 16S RNA or to 30S ribosomal subunits.

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