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PMID: 4140509 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The identification of the eukaryotic ribosomal proteins homologous with Escherichia coli proteins L7 and L12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 4723-6

Stöffler G, Wool IG, Lin A, Rak KH

Abstract

Antibodies were raised against eukaryotic (rat liver) and prokaryotic (E. coli) ribosomal particles and ribosomal proteins. The antisera were characterized and used to determine the identity of the eukaryotic proteins homologous to E. coli L7 and L12. The large subunit of rat liver ribosomes contains two acidic proteins, L40 and L41; they migrate during two-dimensional polyacrylamide gel electrophoresis in a way that mimics the behavior of L7 and L12. Rat liver L40 and L41 were found to be immunologically related to E. coli L7/L12, hence the proteins are likely to share some structural homology.

MeSH Terms
Animals Bacterial Proteins/analysis,immunology Cross Reactions Electrophoresis, Polyacrylamide Gel Epitopes Escherichia coli/analysis Immunodiffusion Liver/ultrastructure Proteins/analysis,immunology Rats Ribosomes/analysis
Chemicals
Bacterial Proteins Epitopes Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stöffler G
Wool I G
Lin A
Rak K H
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.4723
Published
1974-12-00
Pages
4723-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433968
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.4723 PMC Full Text View journal details
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