Isolation and characterization of bacteria that grow on methane and organic compounds as sole sources of carbon and energy.

Journal of bacteriology ·Vol. 120 ·No. 2 ·1974-11-00 ·Pages 955-64

Patt TE, Cole GC, Bland J, Hanson RS

Abstract

Bacteria capable of growth on methane and a variety of complex organic substrates as sole sources of carbon and energy have been isolated. Conditions used to rigorously establish the purity of the cultures are described. One facultative methylotroph has been studied in detail. This organism has peripherally arranged pairs of intracytoplasmic membranes characteristic of obligate methylotrophs. This isolate apparently utilizes the serine pathway of formaldehyde fixation. The location of methane oxidizers in a dimictic lake indicates that these organisms prefer less than saturating levels of dissolved oxygen. Laboratory experiments confirmed the preference of these organisms for atmospheres containing less oxygen than air.

MeSH Terms

Anti-Bacterial Agents/pharmacology Bacteria/enzymology,isolation & purification,ultrastructure Bacterial Proteins/analysis Carbon/metabolism Carbon Radioisotopes Centrifugation, Density Gradient Cesium Chromatography, Gas Culture Media DNA, Bacterial/isolation & purification Escherichia coli/metabolism Glucose/metabolism Gramicidin/pharmacology Methane/metabolism Micrococcus/metabolism Microscopy, Electron Molecular Weight Spectrophotometry Streptomycin/pharmacology Time Factors Tritium Water Microbiology

Chemicals

Anti-Bacterial Agents Bacterial Proteins Carbon Radioisotopes Culture Media DNA, Bacterial Tritium Gramicidin Cesium Carbon Glucose Methane Streptomycin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Patt T E

Cole G C

Bland J

Hanson R S

References (26)

26 references, click to expand

The biosynthesis of serine and glycine in Pseudomonas AM1 with special reference to growth on carbon sources other than C1 compounds.
Biochem J. 1971 Mar;121(5):753-62 PMID: 4329715
Ribulose diphosphate carboxylase from autotrophic microorganisms.
J Bacteriol. 1972 May;110(2):633-42 PMID: 4623310
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
J Mol Biol. 1962 Jun;4:430-43 PMID: 14498379
SINGLE-CELL ISOLATION AND PRESERVATION OF BACTERIAL CULTURES.
Nature. 1963 Aug 24;199:775-7 PMID: 14071187
Hexose phosphate synthese and tricarboxylic acid-cycle enzymes in bacterium 4B6, an obligate methylotroph.
Biochem J. 1972 Aug;128(5):1373-6 PMID: 4643709
A low-viscosity epoxy resin embedding medium for electron microscopy.
J Ultrastruct Res. 1969 Jan;26(1):31-43 PMID: 4887011
Alternative carbon assimilation pathways in methane-utilizing bacteria.
J Gen Microbiol. 1970 Nov;63(3):371-4 PMID: 5516439
Enrichment, isolation and some properties of methane-utilizing bacteria.
J Gen Microbiol. 1970 May;61(2):205-18 PMID: 5476891
Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans.
Biochem J. 1970 Feb;116(4):631-9 PMID: 5435492
[Electron microscopic study on plasmas containing desoxyribonucleic acid. I. Nucleoids of actively growing bacteria].
Z Naturforsch B. 1958 Sep;13B(9):597-605 PMID: 13604673
Commitment to sporulation and induction of glucose-phosphoenolpyruvate-transferase.
Biochim Biophys Acta. 1970 Nov 24;222(2):265-89 PMID: 4992519
BIOLOGY OF BUDDING BACTERIA. 3. FINE STRUCTURE OF RHODOMICROBIUM AND HYPHOMICROBIUM SPP.
J Bacteriol. 1965 Feb;89:503-12 PMID: 14255720
The distribution in the methylobacteria of some key enzymes concerned with intermediary metabolism.
Arch Mikrobiol. 1972;87(4):359-66 PMID: 4404762
Methanol metabolism in pseudomonad C.
J Bacteriol. 1973 Apr;114(1):390-8 PMID: 4349032
Serine as an intermediate in the assimilation of methanol by a Pseudomonas.
Biochim Biophys Acta. 1959 May;33(1):106-10 PMID: 13651189
Glucose-6-phosphate dehydrogenase from Escherichia coli and from a "high-level" mutant.
J Bacteriol. 1972 Apr;110(1):155-60 PMID: 4401601
A methane-dependent coccus, with notes on classification and nomenclature of obligate, methane-utilizing bacteria.
J Bacteriol. 1966 May;91(5):1924-31 PMID: 5937247
Microbial growth on C1 compounds. II. Synthesis of cell constituents by methanol- and formate-grown Pseudomonas AM 1, and methanol-grown Hyphomicrobium vulgare.
Biochem J. 1961 Dec;81:470-80 PMID: 14462405
Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics.
J Bacteriol. 1952 Oct;64(4):489-99 PMID: 12999676
Studies on Pseudomonas methanica (Söhngen) nov. comb.
J Bacteriol. 1956 Nov;72(5):646-59 PMID: 13376510
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Microbial growth on C(1) compounds. 5. Enzyme activities in extracts of Pseudomonas AM1.
Biochem J. 1963 May;87(2):386-96 PMID: 16749008
Aspects of glycine and serine biosynthesis during growth of Pseudomonas AM1 on C compounds.
Biochem J. 1971 Mar;121(5):763-9 PMID: 5113490
THE ISOLATION AND CHARACTERIZATION OF METHANOMONAS METHANOOXIDANS BROWN AND STRAWINSKI.
Can J Microbiol. 1964 Oct;10:791-9 PMID: 14222661
Fine structure of methane and other hydrocarbon-utilizing bacteria.
J Gen Microbiol. 1970 May;61(2):227-32 PMID: 5476893
Metabolism of single carbon compounds.
Annu Rev Microbiol. 1970;24:135-58 PMID: 4929654

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.120.2.955-964.1974

Published

1974-11-00

Pages

955-64

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC245862

Subset

External Links

PubMed source DOI: 10.1128/jb.120.2.955-964.1974 PMC Full Text View journal details

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Isolation and characterization of bacteria that grow on methane and organic compounds as sole sources of carbon and energy.

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