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PMID: 414220 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5350-4

Alwine JC, Kemp DJ, Stark GR

Abstract

We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected by hybridization with 32P-labeled DNA probes followed by autoradiography. This procedure allows detection of specific RNA bands with high sensitivity and low background.

MeSH Terms
Animals Autoradiography/methods Cellulose Chemical Phenomena Chemistry Diazonium Compounds Drosophila melanogaster Electrophoresis, Agar Gel/methods Nucleic Acid Hybridization RNA/analysis RNA, Ribosomal/analysis Solubility
Chemicals
Diazonium Compounds RNA, Ribosomal diazobenzyloxymethyl groups RNA Cellulose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Alwine J C
Kemp D J
Stark G R
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5350
Published
1977-12-00
Pages
5350-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431715
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.12.5350 PMC Full Text View journal details
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