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PMID: 4150649 Published · ppublish English Journal Article

The effects of iron-limited growth on the reduced nicotinamide-adenine dinucleotide dehydrogenase activity and the membrane proteins of Candida utilis mitochondria.

The Biochemical journal ·Vol. 136 ·No. 4 ·1973-12-00 ·Pages 1029-37

Clegg RA, Skyrme JE

Abstract

1. The mitochondrial NADH dehydrogenase (EC 1.6.99.3) of Candida utilis exhibited altered properties when the organism was grown under iron-limited conditions. No suitable acceptor was found for assay of this enzyme from iron-limited cells. 2. Mitochondrial membrane proteins from C. utilis were analysed by polyacrylamide-gel electrophoresis. Compared with glycerol-limited cells, iron limitation resulted in the loss of at least two polypeptides from the mitochondrial membrane. 3. Neither of the polypeptides affected by iron limitation was part of a cytochrome, although one of them was part of the mitochondrial NADH dehydrogenase. 4. Non-haem iron of mitochondrial membranes was released in the presence of sodium dodecyl sulphate, and electrophoresis in solutions of this detergent cannot be used directly to identify iron-sulphur proteins. Non-ionic detergents do not release non-haem iron but nor do they provide a satisfactory system for electrophoretic separation.

MeSH Terms
Candida/cytology,enzymology,metabolism Carbon Radioisotopes Cytochromes Densitometry Electrophoresis, Polyacrylamide Gel Glycerol/pharmacology Growth/drug effects Heme Iron/pharmacology Membranes/metabolism Mitochondria/enzymology,metabolism NADH, NADPH Oxidoreductases/metabolism Peptides/metabolism Proteins/metabolism Sodium Dodecyl Sulfate Tritium
Chemicals
Carbon Radioisotopes Cytochromes Peptides Proteins Tritium Sodium Dodecyl Sulfate Heme Iron NADH, NADPH Oxidoreductases Glycerol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clegg R A
Skyrme J E
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25 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1361029
Published
1973-12-00
Pages
1029-37
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1166053
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1361029 PMC Full Text View journal details
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