Home LiteratureArticle Details
PMID: 4150653 Published · ppublish English Journal Article

Isoenzyme patterns in parenchymal and non-parenchymal cells isolated from regenerating and regenerated rat liver.

The Biochemical journal ·Vol. 136 ·No. 4 ·1973-12-00 ·Pages 947-54

Bonney RJ, Walker PR, Potter VR

Abstract

Parenchymal and non-parenchymal cells were isolated from adult rat liver that had been fully regenerated after a 70% partial hepatectomy. The characteristics of the parenchymal cell preparations from regenerated rat liver indicated that they were a homogeneous population and comparable with parenchymal cells isolated from intact liver. The parenchymal cells from regenerated adult rat liver contain glucokinase, hexokinase, pyruvate kinase type I and aldolase B. The non-parenchymal cells contain hexokinase, pyruvate kinase type III and aldolase B. When cells were isolated at different times of the day from rats on controlled feeding schedules, variation of tyrosine aminotransferase activity and liver glycogen content were observed in the parenchymal cells in keeping with the reported diurnal oscillations found in whole liver extracts. When parenchymal cells were isolated from rats 48 and 72h after partial hepatectomy, different isoenzyme patterns were observed. These cells appeared to synthesize pyruvate kinase type III, a function that was assigned previously to non-parenchymal cells or to foetal rat liver hepatocytes.

MeSH Terms
Animals Electrophoresis Fructose-Bisphosphate Aldolase/metabolism Glucokinase/metabolism Hepatectomy Hexokinase/metabolism Isoenzymes/metabolism Liver/cytology,embryology,enzymology Liver Glycogen/metabolism Liver Regeneration Male Pyruvate Kinase/metabolism Rats Time Factors Tyrosine Transaminase/metabolism
Chemicals
Isoenzymes Liver Glycogen Tyrosine Transaminase Hexokinase Glucokinase Pyruvate Kinase Fructose-Bisphosphate Aldolase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bonney R J
Walker P R
Potter V R
References (30)
30 references, click to expand
  1. The development of hepatic glucokinase in the neonatal rat.
    Biochem J. 1965 Dec;97(3):845-54 PMID: 5883129
  2. Multiple hexokinases of rat tissues. Purification and comparison of soluble forms.
    J Biol Chem. 1966 Aug 10;241(15):3546-60 PMID: 5919684
  3. Crystallization, characterization and metabolic regulation of two types of pyruvate kinase isolated from rat tissues.
    J Biochem. 1967 Jul;62(1):71-91 PMID: 4965281
  4. Systematic oscillations in tyrosine transaminase and other metabolic functions in liver of normal and adrenalectomized rats on controlled feeding schedules.
    J Nutr. 1968 Jun;95(2):207-27 PMID: 4385831
  5. Systematic oscillations in metabolic functions in liver from rats adapted to controlled feeding schedules.
    Fed Proc. 1968 Sep-Oct;27(5):1238-45 PMID: 4386257
  6. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.
    J Cell Biol. 1969 Dec;43(3):506-20 PMID: 4900611
  7. Premature formation of glucokinase in developing rat liver.
    J Biol Chem. 1970 Jun 10;245(11):2779-83 PMID: 4192987
  8. Irreversible fixation of increased level of muscle type aldolase activity appearing in rat liver in the early stage of hepatocarcinogenesis.
    Cancer Res. 1970 Mar;30(3):743-52 PMID: 4317859
  9. The development of L- and M-type aldolases in rat liver.
    Biochim Biophys Acta. 1970 Oct 27;222(1):251-2 PMID: 5474544
  10. Preparation and some properties of isolated rat liver cells.
    Biochim Biophys Acta. 1970 Nov 24;222(2):361-71 PMID: 4321546
  11. Effect of iron loading on non-heme iron compounds in different liver cell populations.
    J Biol Chem. 1971 Feb 25;246(4):1025-31 PMID: 5543667
  12. Biochemical characterization of Kupffer and parenchymal cells isolated from rat liver.
    Exp Cell Res. 1971 Jul;67(1):17-26 PMID: 4398202
  13. Benzypyrene hydroxylase activity in isolated parenchymal and nonparenchymal cells of rat liver.
    J Cell Biol. 1972 Feb;52(2):316-21 PMID: 4333451
  14. Simplified method for isolation of intact avian and rat liver parenchymal cells.
    Biochem Biophys Res Commun. 1971 Oct 15;45(2):421-9 PMID: 4334338
  15. Distribution of L- and M-type pyruvate kinase between parenchymal and Kupffer cells of rat liver.
    Biochim Biophys Acta. 1972 Aug 28;276(2):425-9 PMID: 4626975
  16. Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver.
    Biochem J. 1972 Feb;126(4):1009-23 PMID: 4262895
  17. Preparation of rat liver cells. I. Effect of Ca 2+ on enzymatic dispersion of isolated, perfused liver.
    Exp Cell Res. 1972 Oct;74(2):450-4 PMID: 4343020
  18. Binucleation in mammalian liver. Studies on the control of cytokinesis in vivo.
    Exp Cell Res. 1972 Oct;74(2):455-65 PMID: 4343021
  19. Preparation of single-cell suspensions from normal liver, regenerating liver, and Morris hepatomas 9121 and 5123tc.
    Cancer Res. 1972 Nov;32(11):2568-76 PMID: 4343238
  20. Pyruvate kinase, hexokinase, and aldolase isozymes in rat liver cells in culture.
    In Vitro. 1972 Sep-Oct;8(2):107-14 PMID: 4630783
  21. Comparison of the properties of two forms of pyruvate kinase in rat liver and determination of their separate activities during development.
    Biochem J. 1972 May;127(4):721-31 PMID: 4631223
  22. Glucose-adenosine 5'-triphosphate 6-phosphotransferases of isolated rat liver parenchymal cells.
    Biochem J. 1972 Dec;130(4):1153-5 PMID: 4656800
  23. A rapid method for the isolation of large quantities of rat liver parenchymal cells with high anabolic rates.
    Biochem Biophys Res Commun. 1972 Apr 28;47(2):403-10 PMID: 4350995
  24. Allosteric properties of a pyruvate kinase isoenzyme from rat liver cells in culture.
    J Biol Chem. 1973 Jul 10;248(13):4610-6 PMID: 4736978
  25. Glycolytic isoenzymes and glycogen metabolism in regenerating liver from rats on controlled feeding schedules.
    Biochem J. 1973 Sep;136(1):115-24 PMID: 4772620
  26. Thymidine kinase and deoxyribonucleic acid metabolism in growing and regenerating livers from rats on controlled feeding schedules.
    Biochem J. 1973 Dec;136(4):955-66 PMID: 4786540
  27. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  28. A new method for the preparation of liver cell suspensions.
    Exp Cell Res. 1962 Sep;27:453-67 PMID: 13964289
  29. COMPARATIVE STUDIES OF LIVER AND MUSCLE ALDOLASE. II. IMMUNOCHEMICAL AND CHROMATOGRAPHIC DIFFERENTIATION.
    J Biol Chem. 1963 Oct;238:3280-5 PMID: 14085374
  30. PYRIMIDINE METABOLISM IN TISSUE CULTURE CELLS DERIVED FROM RAT HEPATOMAS. I. SUSPENSION CELL CULTURES DERIVED FROM THE NOVIKOFF HEPATOMA.
    Cancer Res. 1965 May;25:499-508 PMID: 14297488
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1360947
Published
1973-12-00
Pages
947-54
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1166044
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1360947 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2