Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay.

Infection and immunity ·Vol. 19 ·No. 2 ·1978-02-00 ·Pages 540-6

Yolken RH, Wyatt RG, Kim HW, Kapikian AZ, Chanock RM

Abstract

The report describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against the human reovirus-like agent of infantile gastroenteritis (HRVLA). This ELISA system proved to be four times as sensitive as the standard anti-HRVLA fluorescent-antibody assay and ten times as sensitive as the standard anti-HRVLA complement fixation assay. In addition, the ELISA was capable of determining immunoglobulin G (IgG) and IgM subclasses of anti-HRVLA antibody using a single dilution os serum. With this assay, it was discovered that 11 of 21 infected children had anti-HRVLA IgM in their acute sera before the appearance of anti-HRVLA IgG. ELISA is a useful tool in the evaluation of immunological response to HRVLA infection.

MeSH Terms

Adult Antibodies, Viral/biosynthesis Child Complement Fixation Tests Diarrhea, Infantile/immunology Enzyme-Linked Immunosorbent Assay/methods Fluorescent Antibody Technique Gastroenteritis/immunology Humans Immunoenzyme Techniques/methods Immunoglobulin G/biosynthesis Immunoglobulin M/biosynthesis Viruses, Unclassified/immunology

Chemicals

Antibodies, Viral Immunoglobulin G Immunoglobulin M

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Yolken R H

Wyatt R G

Kim H W

Kapikian A Z

Chanock R M

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24 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.19.2.540-546.1978

Published

1978-02-00

Pages

540-6

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC414117

Subset

External Links

PubMed source DOI: 10.1128/iai.19.2.540-546.1978 PMC Full Text View journal details

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Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay.

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