Nucleotide sequence of threonine tRNA from Bacillus subtilis.

Nucleic acids research ·Vol. 5 ·No. 2 ·1978-02-00 ·Pages 537-48

Hasegawa T, Ishikura H

Abstract

A threonine tRNA was purified from Bacillus subtilis W168 by a combined use of column chromatographic systems. The nucleotide sequence was determined to be pG-C-C-G-G-U-G-U-A-G-C-U-C-A-A-U-D-G-G-D(U)-A-G-A-G-C-A-A-C-U-G-A-C-U-mo5U-G-U-t6A-A-psi-C-A-G-U-A-G-m7G-U-U-G-G-G-G-G-T-psi-C-A-A-G-U-C-C-U-C-U-U-G-C-C-G-G-C-A-C-C-AOH, where about 40 % of D20 remained unmodified as U20. It consists of 76 nucleotides including a new minor nucleoside, 5-methoxyuridine (mo5U), which occupies the wobble position of anticodon.

MeSH Terms

Anticodon/analysis Bacillus subtilis/analysis Base Sequence RNA, Bacterial/isolation & purification RNA, Transfer/isolation & purification Threonine Uridine/analogs & derivatives

Chemicals

Anticodon RNA, Bacterial Threonine 5-methoxyuridine RNA, Transfer Uridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hasegawa T

Ishikura H

References (22)

22 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.2.537

Published

1978-02-00

Pages

537-48

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342000

Subset

Databases

GENBANK

K00276

External Links

PubMed source DOI: 10.1093/nar/5.2.537 PMC Full Text View journal details

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Nucleotide sequence of threonine tRNA from Bacillus subtilis.

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