Pseudomonas macauensis sp. nov., Isolated from the Wastewater Treatment Plant in Macau peninsula.

A Gram-stain-negative, aerobic and triclosan-degrading strain W03T, was isolated from the wastewater treatment plant (WWTP) in the Macau peninsula (Macau SAR, China). Based on polyphasic taxonomic analyses, strain W03T was identified as a novel species within the genus Pseudomonas. It grew optimally in Luria-Bertani medium at pH 7.0, with 1% (w/v) NaCl, and at 30℃. Strain W03T demonstrated a high tolerance to NaCl, with the ability to withstand up to 7% (w/v) NaCl. This strain was characterized using 16S rRNA gene sequencing, average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), average amino acid identity (AAI) along with physiological and biochemical tests. Phylogenetic analysis based on 16S rRNA gene and gyrB gene sequences revealed that strain W03T is most closely related to Pseudomonas triclosanedens ZM23T. The genome size of strain W03T was 6.08 Mbp, with a genomic DNA G + C content of 65.1%. OrthoANI and dDDH values between strain W03T and P. triclosanedens ZM23T confirmed that they represent distinct species. Based on the phylogenetic, physiological, and biochemical characteristics, strain W03T is identified as a novel species within the genus Pseudomonas, for which the name Pseudomonas macauensis sp. nov. is proposed.