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PMID: 417092 Published · ppublish English Journal Article

IgM lambda cytoplasmic crystals in three cases of immunocytoma: a clinical, cytochemical, and ultrastructural study.

Journal of clinical pathology ·Vol. 31 ·No. 3 ·1978-03-00 ·Pages 250-8

Feremans WW, Neve P, Caudron M

Abstract

Endoplasmic reticulum-associated crystals were seen in 1-10% of the bone-marrow lymphocytes and in the lymphocytes of the peripheral blood in three cases of immunocytoma. Their crystalline nature and their location in the cisternae of the rough endoplasmic reticulum was proved by ultrastructural study. IgM lambda in the crystals was demonstrated by fluorescent and peroxidase-labelled antibody methods. The crystals did not stain with the PAS reaction, suggesting that the immunoglobulin was not bound to a carbohydrate group. A defect in glucosyltransferase activity with failure to modify the immunoglobulins could explain the absence of the PAS reaction and the accumulation of immunoglobulin in crystalline before reaching the Golgi region.

MeSH Terms
Aged Cytoplasm/immunology Female Fluorescent Antibody Technique Humans Immunoenzyme Techniques Immunoglobulin Light Chains/analysis Immunoglobulin M/analysis Immunoglobulin lambda-Chains/analysis Lymphocytes/immunology,ultrastructure Lymphoma/immunology,ultrastructure Male Microscopy, Electron Middle Aged Plasmacytoma/immunology,ultrastructure
Chemicals
Immunoglobulin Light Chains Immunoglobulin M Immunoglobulin lambda-Chains
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Feremans W W
Neve P
Caudron M
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31 references, click to expand
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Article Info
Journal
Journal of clinical pathology
Abbr.
J Clin Pathol
ISSN
0021-9746
DOI
10.1136/jcp.31.3.250
Published
1978-03-00
Pages
250-8
Language
English
Region
England
NLM ID
0376601
PMCID
PMC1145238
Subset
IM
External Links
PubMed source DOI: 10.1136/jcp.31.3.250 PMC Full Text View journal details
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