A Novel Species Description of Sphingobium agrisoil sp. nov.

Two Gram-stain positive, motile, aerobic, yellowish rod-shaped bacterial strains sgz301303 and sgz301304T were isolated from the paddy soils. The 16S rRNA gene phylogenetic analysis revealed that both strains belong to the genus Sphingobium, and exhibited the highest sequence similarity (98.3%) with reference strain Sphingobium amiense JCM 11777T. The draft genome of sgz301304T has a size of 4.3 Mbp, with DNA G + C content of 64.5%. Whole-genome relatedness analyses showed that the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain sgz301304T and S. amiense JCM 11777T were 82.3% and 25.7%, respectively, both below the accepted species delineation thresholds. The main quinone was Q-10, and the main fatty acids included C14:0 2-OH (11.3%), and Summed Features 8 (46.6%). Phylogenetic, genomic and phenotypic analyses suggest that strain sgz301304T belongs to a novel species within the genus of Sphingobium. Therefore, it is recommended to be named as Sphingobium agrisoil sp. nov. The type strain is sgz301304T (= MCCC 1K09180T = JCM 37704T), respectively.