Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 3 ·1969-07-00 ·Pages 753-8

Klement V, Rowe WP, Hartley JW, Pugh WE

Abstract

Tissue culture cells of a Rous virus-induced rat tumor undergo syncytium formation when placed in contact with mouse embryo cell cultures infected with murine leukemia viruses. This phenomenon can be used as a cytopathic end point for isolating and titrating these viruses in tissue culture. The principle should be applicable to detection of leukemia viruses of other species.

MeSH Terms

Animals Avian Sarcoma Viruses/isolation & purification Biological Assay Cell Line Culture Media Culture Techniques Cytopathogenic Effect, Viral Leukemia Virus, Murine/isolation & purification Methods Mice Rats Staining and Labeling Virus Cultivation

Chemicals

Culture Media

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Klement V

Rowe W P

Hartley J W

Pugh W E

References (5)

5 references, click to expand

Plaque assay of Visna virus using a secondary cellular overlay as an indicator.
Virology. 1967 Jan;31(1):176-8 PMID: 4959706
Biological and serological properties of viral particles from a nonproducer rat neoplasm induced by murine sarcoma virus (Moloney).
J Virol. 1968 Sep;2(9):865-8 PMID: 5725318
Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture.
J Virol. 1969 Feb;3(2):126-32 PMID: 4304442
Demonstration of the absence of infectious Rous virus in rat tumour XC, whose structurally intact cells produce Rous sarcoma when transferred to chicks.
Folia Biol (Praha). 1963 Apr;9:77-81 PMID: 13984556
Presence of chicken tumour virus in the sarcoma of the adult rat inoculated after birth with Rous sarcoma tissue.
Nature. 1960 Jun 18;186:980-1 PMID: 13835975

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.63.3.753

Published

1969-07-00

Pages

753-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223516

Subset

External Links

PubMed source DOI: 10.1073/pnas.63.3.753 PMC Full Text View journal details

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