Relation between deoxyribonucleic acid and intracytoplasmic membranes in Escherichia coli O111a.

Journal of bacteriology ·Vol. 103 ·No. 1 ·1970-07-00 ·Pages 227-37

Altenburg BC, Suit JC

Abstract

The possibility of a relationship between intracytoplasmic membranes and deoxyribonucleic acid (DNA) in Escherichia coli O111a(1) has been investigated. To facilitate this investigation, a simple enzymatic assay for the amount of internal membrane present in a culture was developed. This assay was then used to show that the appearance of intracytoplasmic membranes is accompanied by an increase in the DNA content of the cells. Electron micrographs have confirmed this observation and have shown DNA to be in contact with the intracytoplasmic membranes. Extensive membranes were observed at sites of apparently unsuccessful attempts at cell division. These observations led to the conclusion that the internal membrane formed by strain O111a(1) represents "extra" membrane, which is functional in that it contains sites for DNA replication, but is produced in excess because the organism is somehow defective in its regulation of membrane synthesis.

MeSH Terms

Bacterial Proteins/analysis Cell Division Cell Membrane/enzymology Colorimetry Cytoplasm DNA Replication DNA, Bacterial/analysis,metabolism Escherichia coli/analysis,cytology,growth & development,metabolism Genetics, Microbial Lipids/analysis Malate Dehydrogenase/metabolism Microscopy, Electron Mutation Spectrophotometry Staining and Labeling

Chemicals

Bacterial Proteins DNA, Bacterial Lipids Malate Dehydrogenase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Altenburg B C

Suit J C

References (13)

13 references, click to expand

On the association between DNA and membrane in bacteria.
Proc R Soc Lond B Biol Sci. 1966 Mar 22;164(995):267-78 PMID: 4379516
[Thermosensitive mutants of Escherichia coli K 12. I. Isolation and rapid characterization].
Ann Inst Pasteur (Paris). 1966 Apr;110(4):465-86 PMID: 5325383
Intracytoplasmic membranes in Escherichia coli.
J Bacteriol. 1966 Sep;92(3):780-3 PMID: 4162461
Indentification and localization of the fatty acids in Haemophilus parainfluenzae.
J Bacteriol. 1967 Mar;93(3):1079-88 PMID: 6025415
Procedure for embedding in situ selected cells cultured in vitro.
J Cell Biol. 1967 Oct;35(1):279-83 PMID: 4863049
Properties of the growing point region in the bacterial chromosome.
Biochim Biophys Acta. 1967 Dec 19;149(2):519-31 PMID: 4866437
Association of the nucleus and the membrane of bacteria: a morphological study.
Bacteriol Rev. 1968 Mar;32(1):39-54 PMID: 4869940
Lysis of spheroplasts of Escherichia coli by a non-ionic detergent.
Biochem Biophys Res Commun. 1968 May 10;31(3):438-46 PMID: 4297527
Mesosomes in Escherichia coli.
J Bacteriol. 1969 Jan;97(1):367-75 PMID: 4884819
On the bacterial life sequence.
Cold Spring Harb Symp Quant Biol. 1968;33:809-22 PMID: 4892010
Process of infection with bacteriophage phiX174. XX. Attachment of the parental DNA of bacteriophage phiX174 to a fast-sedimenting cell component.
J Mol Biol. 1968 May 28;34(1):17-29 PMID: 5760452
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.103.1.227-237.1970

Published

1970-07-00

Pages

227-37

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC248060

Subset

External Links

PubMed source DOI: 10.1128/jb.103.1.227-237.1970 PMC Full Text View journal details

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Relation between deoxyribonucleic acid and intracytoplasmic membranes in Escherichia coli O111a.

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