Preservation of normal behavior by enucleated cells in culture.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 3 ·1973-03-00 ·Pages 750-4

Goldman RD, Pollack R, Hopkins NH

Abstract

BHK-21 fibroblasts and BSC-1 epithelial cells were enucleated with cytochalasin B. The enucleated cells were trypsinized and replated; their subsequent behavior was monitored by light optical techniques. The results demonstrate that the information necessary for attachment, spreading, shape formation, pinocytosis, contact inhibition, and cell locomotion is present in enucleated cytoplasm.

MeSH Terms

Animals Cell Fractionation Cell Line Cell Movement Cell Nucleus Cells, Cultured Contact Inhibition Cricetinae Cytochalasin B Cytoplasm Epithelial Cells Fibroblasts Haplorhini Kidney Microscopy, Phase-Contrast Organoids Pinocytosis

Chemicals

Cytochalasin B

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Goldman R D

Pollack R

Hopkins N H

References (11)

11 references, click to expand

Effects of cytochalasins on mammalian cells.
Nature. 1967 Jan 21;213(5073):261-4 PMID: 6067685
Structural aspects of saltatory particle movement.
J Gen Physiol. 1967 Jul;50(6):Suppl:223-39 PMID: 6069914
Birefringent filamentous organelle in BHK-21 cells and its possible role in cell spreading and motility.
Science. 1970 Jul 17;169(3942):286-8 PMID: 4915821
The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine.
J Cell Biol. 1971 Dec;51(3):752-62 PMID: 4942774
Enucleation of mammalian cells with cytochalasin B.
Exp Cell Res. 1972;71(2):480-5 PMID: 5065231
Nuclear-cytoplasmic relationships in human cells in tissue culture. II. The microscopic behavior of enucleate human cell iragments.
Exp Cell Res. 1960 Mar;19:332-42 PMID: 13850771
Observations on the social behaviour of cells in tissue culture. II. Monolayering of fibroblasts.
Exp Cell Res. 1954 May;6(2):293-306 PMID: 13173482
Interference microscope studies of cell contacts in tissue culture.
Exp Cell Res. 1958 Oct;15(2):332-45 PMID: 13597891
The movements of fibrocytes.
Exp Cell Res. 1961;Suppl 8:54-73 PMID: 13682921
Nuclear-cytoplasmic relationships in human cells in tissue culture. IV. A study of some aspects of nucleic acid and protein metabolism in enucleate cells.
Biochim Biophys Acta. 1960 Dec 4;45:82-6 PMID: 13706654
Formation of anucleate and multinucleate cells in normal and SV 40 transformed WI-38 by cytochalasin B.
Exp Cell Res. 1972 Sep;74(1):187-94 PMID: 4342183

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.3.750

Published

1973-03-00

Pages

750-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433350

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.3.750 PMC Full Text View journal details

Analysis Services

Preservation of normal behavior by enucleated cells in culture.

Contact

WeChat Official Account