The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa.

The Biochemical journal ·Vol. 132 ·No. 2 ·1973-02-00 ·Pages 141-54

Midgley M, Dawes EA

Abstract

The methyl alpha-glucoside-transport system of Pseudomonas aeruginosa has been characterized with respect to its specificity, energy-dependence, kinetics and regulation. The uptake of glucose involved two components, one of which transported glucose (K(m)=8mum) and methyl alpha-glucoside (K(m)=2.8mm) whereas the other was more complex, involving the extracellular activity of glucose dehydrogenase. Mutants defective in this enzyme have been isolated and characterized. The methyl alpha-glucoside-glucose-transport system was repressed when the organism was grown in the absence of glucose; the induction of this transport system by glucose, and its activity once induced, were inhibited by products of citrate metabolism.

MeSH Terms

Azides/pharmacology Biological Transport Biological Transport, Active Carbon Isotopes Cell Membrane/metabolism Chromatography, Ion Exchange Chromatography, Paper Enzyme Induction Enzyme Repression Escherichia coli/metabolism Glucose/metabolism,pharmacology Glucose Oxidase/metabolism Glycosides/metabolism Kinetics Methanol/metabolism Pseudomonas aeruginosa/cytology,drug effects,enzymology,metabolism Species Specificity Time Factors

Chemicals

Azides Carbon Isotopes Glycosides Glucose Oxidase Glucose Methanol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Midgley M

Dawes E A

References (13)

13 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1320141

Published

1973-02-00

Pages

141-54

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177574

Subset

External Links

PubMed source DOI: 10.1042/bj1320141 PMC Full Text View journal details

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The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa.

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