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PMID: 4199334 Published · ppublish English Journal Article

Simplified method for producing pyocins from Pseudomonas aeruginosa.

Applied microbiology ·Vol. 26 ·No. 1 ·1973-07-00 ·Pages 120-1

Jones LF, Pinto BV, Thomas ET, Farmer JJ

Abstract

Good yields of pyocin are obtained when Pseudomonas aeruginosa is grown in Trypticase soy broth (without glucose) (BBL) plus 1% potassium nitrate. As a result, pyocin production for routine epidemiological typing can be done in screw-cap tubes, without mechanical agitation or mitomycin C induction.

MeSH Terms
Bacteriocins/analysis,biosynthesis Bacteriological Techniques Culture Media Methods Nitrates/metabolism Nitrites/biosynthesis Potassium Pseudomonas aeruginosa/classification,growth & development,metabolism
Chemicals
Bacteriocins Culture Media Nitrates Nitrites Potassium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jones L F
Pinto B V
Thomas E T
Farmer J J
References (6)
6 references, click to expand
  1. Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity of pyocin and bacteriophage.
    Appl Microbiol. 1969 Nov;18(5):760-5 PMID: 4984201
  2. Typing of Pseudomonas pyocyanea by pyocine production.
    J Pathol Bacteriol. 1966 Apr;91(2):339-45 PMID: 4958434
  3. The aerobic pseudomonads: a taxonomic study.
    J Gen Microbiol. 1966 May;43(2):159-271 PMID: 5963505
  4. Pyocine typing of clinical strains of Pseudomonas aeruginosa.
    Appl Microbiol. 1969 Feb;17(2):293-6 PMID: 4975658
  5. Epidemiology of Pseudomonas aeruginosa in a burns hospital: surveillance by a combined typing system.
    Appl Microbiol. 1972 Aug;24(2):219-25 PMID: 4627230
  6. THE PRODUCTION AND INACTIVATION OF PYOCINES.
    J Hyg (Lond). 1963 Dec;61:431-41 PMID: 14099022
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.26.1.120-121.1973
Published
1973-07-00
Pages
120-1
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC379729
Subset
IM
External Links
PubMed source DOI: 10.1128/am.26.1.120-121.1973 PMC Full Text
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