Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay.

Applied microbiology ·Vol. 26 ·No. 1 ·1973-07-00 ·Pages 43-8

Jorgensen JH, Smith RF

Abstract

Limulus amoebocyte lysate was prepared from a total of 180 crabs during 1971 and 1972 by using a slightly modified lysate preparation procedure. Marked variability of lysate potency was noted both years. In addition, lysate quality appeared diminished in 1972 as compared with 1971. Different lysate batches were evaluated for potency by using a variety of endotoxin preparations. Variations in batch potencies were observed, but little variation in reactivity among different endotoxin preparations was noted. Use of potent lysate batches allowed detection of endotoxin concentrations as low as 100 pg/ml. No endotoxinlike activity was observed from 11 different strains of yeast by use of the Limulus assay.

MeSH Terms

Animals Bacteria/analysis Brachyura Endotoxins/analysis Escherichia coli/analysis Evaluation Studies as Topic Hemolymph Methods Pseudomonas aeruginosa/analysis Salmonella enteritidis/analysis Seasons Serratia marcescens/analysis Tissue Extracts/isolation & purification,standards Yeasts/analysis

Chemicals

Endotoxins Tissue Extracts

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jorgensen J H

Smith R F

References (12)

12 references, click to expand

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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.26.1.43-48.1973

Published

1973-07-00

Pages

43-8

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC379714

Subset

External Links

PubMed source DOI: 10.1128/am.26.1.43-48.1973 PMC Full Text

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Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay.

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