Home LiteratureArticle Details
PMID: 4200542 Published · ppublish English Journal Article

Effect of heat-labile factors on the neutralization of vaccinia virus by human.

Infection and immunity ·Vol. 8 ·No. 4 ·1973-10-00 ·Pages 582-9

Takabayashi K, McIntosh K

Abstract

The effect of unheated guinea pig and human prevaccination serum on the neutralization of vaccinia virus was studied. Enhancement of neutralization was found in all sera containing antibody and was more marked (20- to 150-fold) in sera obtained in the first weeks after primary vaccination than in sera from immune adults or cord sera (4- to 25-fold). The enhancing factor was thought to be complement because it was destroyed by heating and ethylenediaminetetraacetic acid treatment. The fresh serum-enhanced neutralization test represents a highly sensitive and specific test which can be applied to measure low levels of vaccinia antibody not detectable by other means.

MeSH Terms
Adult Animals Antibodies, Viral Antigens, Viral Child, Preschool Edetic Acid Guinea Pigs/immunology Hot Temperature Humans Immune Sera/pharmacology Neutralization Tests Smallpox Vaccine Vaccines, Attenuated Vaccinia virus/immunology
Chemicals
Antibodies, Viral Antigens, Viral Immune Sera Smallpox Vaccine Vaccines, Attenuated Edetic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Takabayashi K
McIntosh K
References (23)
23 references, click to expand
  1. Evaluation of the demonstration of complement-requiring neutralizing antibody as a means for early diagnosis of herpes virus infections.
    J Immunol. 1966 Feb;96(2):196-203 PMID: 4286752
  2. Rubella virus neutralization by plaque reduction.
    Proc Soc Exp Biol Med. 1967 Jan;124(1):167-72 PMID: 6017761
  3. Experiences with improvement of the neutralization reaction with vaccinia virus in chick fibroblast cultures.
    J Hyg Epidemiol Microbiol Immunol. 1966;10(4):488-98 PMID: 6006816
  4. Neutralization of an infectious herpes simplex virus-antibody complex by anti-gamma-globulin.
    Proc Natl Acad Sci U S A. 1966 Aug;56(2):447-51 PMID: 4165517
  5. Herpes simplex neutralizing antibody--quantitation of the complement-dependent fraction in different phases of adult human infection.
    J Immunol. 1967 Jul;99(1):214-22 PMID: 4291444
  6. Heterogeneity in the properties of 7 S and 19S rabbit-neutralizing antibodies to herpes simplex virus.
    J Immunol. 1968 Mar;100(3):586-93 PMID: 4296105
  7. Effect of complement and viral filtration on the neutralization of respiratory syncytial virus.
    Appl Microbiol. 1968 Jul;16(7):1076-80 PMID: 5664124
  8. The morphological and biological effects of various antisera on avian infectious bronchitis virus.
    J Gen Virol. 1968 Jul;3(1):97-102 PMID: 5692879
  9. Smallpox vaccination of eczema patients with attenuated live vaccinia virus.
    Yale J Biol Med. 1968 Aug;41(1):1-12 PMID: 5683825
  10. Complement requirement of the neutralizing antibody appearing after immunization with smallpox vaccine.
    Jpn J Microbiol. 1968 Jun;12(2):256-9 PMID: 5303512
  11. Complement components required for virus neutralization by early immunoglobulin antibody.
    Proc Natl Acad Sci U S A. 1969 Oct;64(2):520-7 PMID: 4982356
  12. Neutralization of sensitized virus by purified components of complement.
    Proc Natl Acad Sci U S A. 1970 Mar;65(3):528-35 PMID: 4315612
  13. Immune virolysis: effect of antibody and complement on C-type RNA virus.
    Science. 1970 Jun 19;168(3938):1478-80 PMID: 4315656
  14. [Enhancement of neutralization of vaccinia virus by antiglobulin].
    Arch Gesamte Virusforsch. 1970;31(1):28-32 PMID: 5312165
  15. Labile serum factor and its effect on arbovirus neutralization.
    Appl Microbiol. 1971 Jan;21(1):79-83 PMID: 5540674
  16. Herpesvirus neutralization: the role of complement.
    J Immunol. 1971 Nov;107(5):1235-42 PMID: 5000723
  17. Complement-requiring neutralizing antibodies in hyperimmune sera to human cytomegaloviruses.
    J Immunol. 1971 Dec;107(6):1618-30 PMID: 4330457
  18. Enhancement of hemagglutination inhibition by complement.
    Infect Immun. 1972 Dec;6(6):945-51 PMID: 4674079
  19. Two heat-labile factors in normal sera which neutralize variola virus.
    Br J Exp Pathol. 1952 Dec;33(6):529-36 PMID: 13009039
  20. Thermolabile serum factor influencing the neutralisation of adenovirus.
    Lancet. 1963 Jan 12;1(7272):116-7 PMID: 13979525
  21. THE CYCLE OF MULTIPLICATION OF VACCINIA VIRUS IN EARLE'S STRAIN L CELLS. I. UPTAKE AND PENETRATION.
    Virology. 1964 Nov;24:278-94 PMID: 14227031
  22. STUDIES ON THE NEUTRALIZATION OF HERPES SIMPLEX VIRUS. I. APPEARANCE OF NEUTRALIZING ANTIBODIES HAVING DIFFERENT GRADES OF COMPLEMENT REQUIREMENT.
    Virology. 1965 May;26:44-53 PMID: 14295231
  23. STUDIES ON THE NEUTRALIZATION OF HERPES SIMPLEX VIRUS. 3. MECHANISM OF THE ANTIBODY-POTENTIATING ACTION OF COMPLEMENT.
    Virology. 1965 May;26:61-72 PMID: 14293314
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.8.4.582-589.1973
Published
1973-10-00
Pages
582-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC422895
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.8.4.582-589.1973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2