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PMID: 4200722 Published · ppublish English Journal Article

Differentiation in Nostoc muscorum: nitrogenase is synthesized in heterocysts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 10 ·1973-10-00 ·Pages 2727-31

Fleming H, Haselkorn R

Abstract

Cellular differentiation can be observed in certain filamentous blue-green algae after transfer of the cells from medium containing NH(4) (+) or NO(3) (-) to nitrogen-free medium. The appearance of differentiated cells (heterocysts) is accompanied by an increase in the activity of nitrogenase, an enzyme complex that reduces N(2) to NH(3). We have separated vegetative cells from heterocysts in differentiated filaments of Nostoc muscorum, and analyzed their proteins by polyacrylamide-gel electrophoresis. The pattern of incorporation of (35)SO(4) (-) into proteins of the two cell types during differentiation indicates that the increase in nitrogenase activity is due to the de novo synthesis of nitrogenase proteins predominantly, if not exclusively, in heterocysts.

MeSH Terms
Ammonia/pharmacology Cell Differentiation Cell Fractionation Cyanobacteria/cytology,enzymology Electrophoresis, Polyacrylamide Gel Enzyme Induction/drug effects Nitrogenase/analysis,biosynthesis Plant Proteins/biosynthesis Sulfates/metabolism Sulfur Radioisotopes
Chemicals
Plant Proteins Sulfates Sulfur Radioisotopes Ammonia Nitrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fleming H
Haselkorn R
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.10.2727
Published
1973-10-00
Pages
2727-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427096
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.10.2727 PMC Full Text View journal details
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