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PMID: 4200724 Published · ppublish English Journal Article

Strychnine binding associated with glycine receptors of the central nervous system.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 10 ·1973-10-00 ·Pages 2832-6

Young AB, Snyder SH

Abstract

[(3)H]Strychnine binds to synaptic-membrane fractions of the spinal cord in a selective fashion, indicating an interaction with postsynaptic glycine receptors. Displacement of strychnine by glycine and other amino acids parallels their glycine-like neurophysiologic activity. The regional localization of strychnine binding in the central nervous system correlates closely with endogenous glycine concentrations. In subcellular fractionation experiments, strychnine binding is most enhanced in synaptic-membrane fractions. Strychnine binding is saturable, with affinity constants for glycine and strychnine of 10 and 0.03 muM, respectively.

MeSH Terms
Amino Acids/metabolism Animals Binding, Competitive Brain/metabolism Brain Stem/cytology,metabolism Cell Fractionation Cell Nucleus/metabolism Glycine/metabolism Haplorhini In Vitro Techniques Kinetics Macaca Male Microsomes/metabolism Mitochondria/metabolism Rats Receptors, Drug Spinal Cord/cytology,metabolism Strychnine/metabolism Synaptic Membranes/metabolism Tritium
Chemicals
Amino Acids Receptors, Drug Tritium Strychnine Glycine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Young A B
Snyder S H
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.10.2832
Published
1973-10-00
Pages
2832-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427119
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.10.2832 PMC Full Text View journal details
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