Replication of dengue virus type 2 in Aedes albopictus cell culture.

Journal of virology ·Vol. 12 ·No. 2 ·1973-08-00 ·Pages 275-83

Sinarachatanant P, Olson LC

Abstract

The replication of type 2 dengue (D-2) virus in Aedes albopictus (Aal) mosquito cell cultures differed from that in vertebrate (LLC-MK2) rhesus monkey kidney cells. Virus readily replicated in Aal cells at either 30 or 37 C, but had no apparent effect on the host cell. Persistent infection was established with continual virus production for at least 6 months, although the virulence of progeny virus for both suckling mice and LLC-MK2 cells became attenuated. Density gradient analysis of infected Aal cell supernatant products indicated that only complete virus was released, in contrast to infected LLC-MK2 cells which also released incomplete virus. The surface antigens of the virus produced in Aal cells appeared to be considerably modified in that antiserum to vertebrate cell-produced D-2 virus did not block hemagglutination, whereas anti-Aal cell antiserum did. Virus infectivity could be neutralized by the antiserum to D-2 virus grown in vertebrate cells, however. Virus produced in LLC-MK2 cells did not demonstrate a similar host-cell modification. These results may reflect a difference in the mechanism by which D-2 virus matures in Aal cells.

MeSH Terms

Aedes Animals Antigens, Viral/analysis Cell Line Centrifugation, Density Gradient Complement Fixation Tests Culture Techniques Dengue Virus/growth & development,immunology,pathogenicity Haplorhini Hemagglutination Inhibition Tests Hemagglutination Tests Hemagglutinins, Viral/analysis Kidney Macaca Mice Neutralization Tests Temperature Viral Plaque Assay Virulence Virus Replication

Chemicals

Antigens, Viral Hemagglutinins, Viral

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sinarachatanant P

Olson L C

References (25)

25 references, click to expand

Studies on the nature of dengue viruses. I. Correlation of particle density, infectivity, and RNA content of type 2 virus.
Virology. 1965 Sep;27(1):103-12 PMID: 5828097
Studies on the nature of dengue viruses. II. Characterization of viral RNA and effects of inhibitors of RNA synthesis.
Virology. 1966 Oct;30(2):303-12 PMID: 5919232
A plaque reduction test for dengue virus neutralizing antibodies.
J Immunol. 1967 Aug;99(2):285-90 PMID: 6031202
Establishment of carrier cultures of Aedes albopictur cell line infected with arboviruses.
Indian J Med Res. 1968 Jun;56(6):812-4 PMID: 5680414
Susceptibility of Aedes albopictus and Aedes aegypti cell lines to infection by Arbo and other viruses.
Indian J Med Res. 1968 Jun;56(6):815-20 PMID: 5680415
A study on the cytopathic effect of arboviruses on cultures from Aedes albopictus cell line.
Indian J Med Res. 1969 Feb;57(2):339-48 PMID: 5771694
Syncytia formation of mosquito cell cultures mediated by type 2 dengue virus.
Virology. 1969 Jul;38(3):482-5 PMID: 5799585
Isolation of Dengue viruses in Aedes albopictus cell cultures.
Bull World Health Organ. 1969;40(6):982-3 PMID: 5307611
Studies on the nature of dengue viruses. IV. The structural proteins of type 2 dengue virus.
Virology. 1969 Nov;39(3):426-38 PMID: 5390795
Sequential dengue virus infections in the white-handed gibbon (Hylobates lar).
Am J Trop Med Hyg. 1970 Jan;19(1):94-102 PMID: 5416293
Proteins specified by herpes simplex virus. 3. Viruses differing in their effects on the social behavior of infected cells specify different membrane glycoproteins.
Proc Natl Acad Sci U S A. 1970 Apr;65(4):865-71 PMID: 4314902
Arbovirus replication in mosquito cell lines (Singh) grown in monolayer or suspension culture.
Proc Soc Exp Biol Med. 1970 May;134(1):356-61 PMID: 5423526
Molecular size and charge relationships of the soluble complement-fixing antigens of dengue viruses.
Virology. 1970 Jul;41(3):569-72 PMID: 4912826
Physical and biological properties of dengue-2 virus and associated antigens.
J Virol. 1970 Apr;5(4):524-32 PMID: 4195055
Dengue virions and antigens in brain and serum of infected mice.
J Virol. 1970 Oct;6(4):500-6 PMID: 5497897
Immunological and biophysical separation of dengue-2 antigens.
J Virol. 1971 Jan;7(1):15-23 PMID: 5543428
Comparison of three methods used to isolate dengue virus type 2.
Appl Microbiol. 1971 Dec;22(6):1100-3 PMID: 5002896
Double-stranded RNA in hamster, chick, and mosquito cells infected with Sindbis virus.
Virology. 1972 Jan;47(1):122-32 PMID: 4550788
Arthropod tissue culture in the study of arboviruses and rickettsiae: a review.
Curr Top Microbiol Immunol. 1971;55:113-26 PMID: 4401361
Glycolipid nature of the complement-fixing host cell antigen of vesicular stomatitis virus.
J Gen Virol. 1972 Jun;15(3):243-5 PMID: 4339184
Sporozoite and normal salivary gland induced immunity in malaria.
Nature. 1972 Aug 11;238(5363):341 PMID: 4403942
Change involving a viral membrane glycoprotein during morphogenesis of group B arboviruses.
Virology. 1972 Dec;50(3):906-11 PMID: 4629690
Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses.
Am J Trop Med Hyg. 1958 Sep;7(5):561-73 PMID: 13571577
The significance of the viral carrier state in tissue culture systems.
Prog Med Virol. 1958;1:36-58 PMID: 13579004
The antibody response to smallpox vaccination as measured by a tissue culture plaque method.
J Immunol. 1960 Sep;85:275-83 PMID: 13718978

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.12.2.275-283.1973

Published

1973-08-00

Pages

275-83

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356621

Subset

External Links

PubMed source DOI: 10.1128/JVI.12.2.275-283.1973 PMC Full Text View journal details

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Replication of dengue virus type 2 in Aedes albopictus cell culture.

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