3H-colcicine binding. Failure to detect any binding to soluble proteins from various lower organisms.

Animals Ascomycota/metabolism Binding Sites Brain/metabolism Chlamydomonas/metabolism Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Colchicine/metabolism Electrophoresis, Polyacrylamide Gel Female Fungal Proteins/metabolism Kinetics Mice Molecular Weight Nerve Tissue Proteins/metabolism Ovum Plant Proteins/metabolism Plants/metabolism Protein Binding Receptors, Drug Sea Urchins/metabolism Species Specificity Spectrophotometry, Ultraviolet Sulfur Radioisotopes Tetrahymena pyriformis/metabolism Time Factors Tritium

Fungal Proteins Nerve Tissue Proteins Plant Proteins Receptors, Drug Sulfur Radioisotopes Tritium Colchicine