Isolation and characterization of the proenzyme form of the C1s subunit of the first complement component.

Amino Acids/analysis Animals Buffers Cellulose Centrifugation, Density Gradient Chromatography, Gel Chromatography, Ion Exchange Complement System Proteins/isolation & purification Dithiothreitol Electrophoresis, Polyacrylamide Gel Enzyme Activation Humans Hydrogen-Ion Concentration Immune Sera Immunodiffusion Immunoelectrophoresis Iodine Radioisotopes Molecular Weight Proteins/analysis Rabbits/immunology Sodium Dodecyl Sulfate Ultrafiltration

Amino Acids Buffers Immune Sera Iodine Radioisotopes Proteins Sodium Dodecyl Sulfate Cellulose Complement System Proteins Dithiothreitol