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PMID: 4205196 Published · ppublish English Journal Article

Regulation of aconitase synthesis in Bacillus subtilis: induction, feedback repression, and catabolite repression.

Journal of bacteriology ·Vol. 117 ·No. 3 ·1974-03-00 ·Pages 1295-305

Ohné M

Abstract

The synthesis of aconitase in Bacillus subtilis wild-type and different citric acid cycle mutants has been studied and the influence of various growth conditions examined. Aconitase is induced by citrate and precursors of citrate and repressed by glutamate. Induction and repression counteract each other, and at equimolar concentrations of citrate and glutamate, aconitase synthesis is unaffected. Induction by citrate can partly overcome catabolite repression of aconitase. Isocitrate dehydrogenase show endogenous induction of aconitase due to citrate accumulation. Leaky mutants defective in citrate synthase and aconitase cannot be induced by citrate, which indicates that they carry a regulatory mutation. The complex regulation of aconitase is discussed with reference to the participation of this enzyme in glutamate biosynthesis and energy metabolism.

MeSH Terms
Aconitate Hydratase/biosynthesis Aspartic Acid/metabolism Bacillus subtilis/enzymology,metabolism Cell-Free System Citrate (si)-Synthase/biosynthesis Citrates/metabolism Culture Media Enzyme Induction Enzyme Repression Feedback Fumarates/biosynthesis Genetic Complementation Test Glucose/metabolism Glutamates/metabolism Hydro-Lyases/biosynthesis Isocitrate Dehydrogenase/biosynthesis Ketoglutarate Dehydrogenase Complex/biosynthesis Mutation Succinate Dehydrogenase/biosynthesis
Chemicals
Citrates Culture Media Fumarates Glutamates Aspartic Acid Isocitrate Dehydrogenase Ketoglutarate Dehydrogenase Complex Succinate Dehydrogenase Citrate (si)-Synthase Hydro-Lyases Aconitate Hydratase Glucose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Ohné M
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26 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.117.3.1295-1305.1974
Published
1974-03-00
Pages
1295-305
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246613
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.117.3.1295-1305.1974 PMC Full Text View journal details
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