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PMID: 4205593 Published · ppublish English Journal Article

In vitro synthesis of ribosomal proteins directed by Escherichia coli DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 2 ·1974-02-00 ·Pages 446-50

Kaltschmidt E, Kahan L, Nomura M

Abstract

In vitro synthesis of a number of E. coli 30S ribosomal proteins has been demonstrated in a cell-free system consisting of ribosomes, initiation factors, RNA polymerase, a fraction containing soluble enzymes and factors, and E. coli DNA. DNA-dependent synthesis of the following 30S proteins has been demonstrated: S4, S5, S7, S8, S9, S10, S13, S14, S16, S19, and S20.

MeSH Terms
Bacterial Proteins/biosynthesis,isolation & purification Cell-Free System Coliphages DNA, Bacterial/metabolism DNA, Viral/metabolism DNA-Directed RNA Polymerases/metabolism Deoxyribonucleases/metabolism Electrophoresis Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism Immunodiffusion Methionine/metabolism Peptide Initiation Factors Peptides/analysis Radioimmunoassay Ribosomes/metabolism Rifampin Sulfur Radioisotopes Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Viral Peptide Initiation Factors Peptides Sulfur Radioisotopes Methionine DNA-Directed RNA Polymerases Deoxyribonucleases Rifampin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kaltschmidt E
Kahan L
Nomura M
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.2.446
Published
1974-02-00
Pages
446-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388023
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.2.446 PMC Full Text View journal details
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