Detachment and chemical characterization of the regularly arranged subunits from the surface of an Acinetobacter.

Journal of bacteriology ·Vol. 118 ·No. 2 ·1974-05-00 ·Pages 654-62

Thornley MJ, Thorne KJ, Glauert AM

Abstract

Acinetobacter sp. strain MJT/F5/199A carries an array of tetragonally arranged subunits on its outer surface. The subunits can be detached from isolated cell walls by incubation with 1 M urea or by washing with water after treatment with 10 mM ethylenediaminetetraacetic acid or ethyleneglycol-bis(beta-aminoethylether)N,N'-tetraacetic acid. After removal of the urea, they reaggregate into the same ordered array at air-water interfaces in the presence of MgCl(2). The detached subunits were characterized as an acidic protein of molecular weight 65,000. They represent one-fifth of the total cell wall protein.

MeSH Terms

Alcaligenes/analysis,cytology Amino Acids/analysis Bacterial Proteins/analysis,isolation & purification Cell Fractionation Cell Wall/analysis Chromatography, DEAE-Cellulose Chromatography, Gel Edetic Acid Electrophoresis, Disc Guanidines Magnesium Methods Microscopy, Electron Molecular Weight Urea

Chemicals

Amino Acids Bacterial Proteins Guanidines Urea Edetic Acid Magnesium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Thornley M J

Thorne K J

Glauert A M

References (10)

10 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.118.2.654-662.1974

Published

1974-05-00

Pages

654-62

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC246801

Subset

External Links

PubMed source DOI: 10.1128/jb.118.2.654-662.1974 PMC Full Text View journal details

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Detachment and chemical characterization of the regularly arranged subunits from the surface of an Acinetobacter.

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