Percent charging of transfer ribonucleic acid and levels of ppGpp and pppGpp in dormant and germinated spores of Bacillus megaterium.
The levels of transfer ribonucleic acids (tRNAs) specific for 14 amino acids were almost identical in dormant spores and in spores germinated from 6 to 75 min. Germinated spore tRNAs specific for all amino acids tested were between 63 and 93% charged, and there was no significant change in this value from 6 to 75 min of germination. In contrast, tRNAs isolated from dormant spores specific for nine different amino acids were almost completely(>93%) uncharged. However, some dormant spore tRNAs, i.e., those for arginine, histidine, isoleucine, and valine, showed significant (21 to 72%) levels of aminoacylation. Dormant spores contained no detectable guanosine penta- (pppGpp), tetra- (ppGpp), or triphosphate (GTP). However, these nucleotides appeared in the first minutes of germination, and thereafter all increased in parallel with a ratio of pppGpp plus ppGpp to GTP of 0.07 to 0.11, which is characteristic of unstarved vegetative cells.
25 references, click to expand
-
A role of aminoacyl-tRNA in the regulation of protein breakdown in Escherichia coli.Proc Natl Acad Sci U S A. 1971 Feb;68(2):362-6 PMID: 5277086
-
Alteration of valyl-sRNA during sporulation of bacillus subtilis.Proc Natl Acad Sci U S A. 1966 Mar;55(3):564-71 PMID: 4957526
-
Amino acid control of messenger ribonucleic acid synthesis in Bacillus subtilis.J Biol Chem. 1972 Apr 25;247(8):2289-94 PMID: 4623129
-
Energy charge and protein synthesis. Control of aminoacyl transfer ribonucleic acid synthetases.J Biol Chem. 1970 Jan 25;245(2):450-2 PMID: 4904484
-
Nature of deoxyribonucleic acid synthesis and its relationship to protein synthesis during outgrowth of Bacillus cereus T.J Bacteriol. 1972 Feb;109(2):606-15 PMID: 4621681
-
DRY RUPTURE OF BACTERIAL SPORES.J Bacteriol. 1963 Mar;85:720-1 PMID: 14042959
-
The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a nucleotide associated with the stringent response.J Biol Chem. 1970 May 10;245(9):2309-18 PMID: 4315151
-
Variations in activity of aminoacyl-tRNA synthetases as a function of development in Bacillus subtilis.Arch Biochem Biophys. 1973 Feb;154(2):691-5 PMID: 4632423
-
The control of ribonucleic acid synthesis in Escherichia coli. IV. Relevance of unusual phosphorylated compounds from amino acid-starved stringent strains.J Biol Chem. 1969 Jun 25;244(12):3133-41 PMID: 4893338
-
Enzymatic modification of proteins. 3. Purification and properties of a leucyl, phenylalanyl transfer ribonucleic acid protein transferase from Escherichia coli.J Biol Chem. 1970 Apr 25;245(8):2066-73 PMID: 4909560
-
Characterization of altered forms of glycyl transfer ribonucleic acid synthetase and the effects of such alterations on aminoacyl transfer ribonucleic acid synthesis in vivo.J Bacteriol. 1970 Apr;102(1):204-12 PMID: 4908672
-
Biochemical studies of bacterial sporulation and germination. XI. Protein-synthesizing systems from vegetative cells and spores of Bacillus megaterium.J Biol Chem. 1968 Oct 10;243(19):5117-25 PMID: 4971349
-
Biochemical studies of bacterial sporulation and germination. X. Ribosomes and nucleic acids of vegetative cells and spores of Bacillus megaterium.J Biol Chem. 1968 Oct 10;243(19):5110-6 PMID: 4971348
-
Stimulation by growth hormone and dexamethasone of labeled cyclic adenosine 3',5'-monophosphate accumulation by white fat cells.J Biol Chem. 1970 Mar 10;245(5):1101-7 PMID: 4313703
-
Biochemical studies of bacterial sporulation and germination. XVII. Sulfhydryl and disulfide levels in dormancy and germination.J Bacteriol. 1969 Dec;100(3):1155-60 PMID: 4982889
-
Absence of 3'-terminal residues from transfer ribonucleic acid of dormant spores of Bacillus megaterium.J Bacteriol. 1974 Jan;117(1):126-32 PMID: 4202992
-
Biochemical studies of bacterial sporulation and germination. 23. Nucleotide metabolism during spore germination.J Biol Chem. 1970 Jul 25;245(14):3645-52 PMID: 4990475
-
Intracellular condition of Escherichia coli transfer RNA.Proc Natl Acad Sci U S A. 1966 Apr;55(4):839-46 PMID: 5327069
-
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
-
Roles of amino acid activating enzymes in cellular physiology.Bacteriol Rev. 1966 Dec;30(4):701-19 PMID: 5342516
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
Polyamine levels during growth, sporulation, and spore germination of Bacillus megaterium.J Bacteriol. 1974 Mar;117(3):1171-7 PMID: 4205192
-
Histidine regulation in Salmonella typhimurium. XI. The percentage of transfer RNA His charged in vivo and its relation to the repression of the histidine operon.J Mol Biol. 1972 Apr 28;66(1):131-42 PMID: 4339187
-
Biochemical studies of bacterial sporulation and germination. XXII. Energy metabolism in early stages of germination of Bacillus megaterium spores.J Biol Chem. 1970 Jul 25;245(14):3637-44 PMID: 4394282
-
Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes.Proc Natl Acad Sci U S A. 1973 May;70(5):1564-8 PMID: 4576025
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
