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PMID: 4209883 Published · ppublish English Journal Article

Synthesis of von Willebrand factor by cultured human endothelial cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 5 ·1974-05-00 ·Pages 1906-9

Jaffe EA, Hoyer LW, Nachman RL

Abstract

Cultured human endothelial cells synthesize and secrete a protein(s) which has Factor VIII antigen but which lacks Factor VIII clot-promoting activity (J. Clin. Invest. 52, 2757-2764, 1973). Von Willebrand factor activity has been identified in medium from cultured human endothelial cells. This activity was demonstrated by the ability to correct the defect in platelet adhesiveness of blood obtained from patients with von Willebrand's disease. This activity also supported ristocetin-induced aggregation of washed normal human platelets. The von Willebrand factor activity from cultured endothelial cells has physicochemical and immunologic properties like those of the von Willebrand factor activity and the Factor VIII antigen present in human plasma and the Factor VIII antigen synthesized by human endothelial cells in vitro. Rabbit antibody to chromatographic fractions containing endothelial cell von Willebrand factor inhibits the platelet retention of normal blood in glass bead columns.

MeSH Terms
Animals Antigen-Antibody Reactions Biological Assay Blood Platelets Cells, Cultured Endothelium/metabolism Factor VIII/analysis,biosynthesis Humans Immunodiffusion Rabbits/immunology
Chemicals
Factor VIII
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jaffe E A
Hoyer L W
Nachman R L
References (21)
21 references, click to expand
  1. Platelet adhesiveness in von Willebrand's disease.
    Am J Clin Pathol. 1969 Jul;52(1):69-77 PMID: 5306206
  2. Ultrastructural basis of experimental thrombocytopenic purpura.
    Arch Pathol. 1970 Sep;90(3):197-205 PMID: 5465553
  3. Reversible decrease in platelet retention by glass bead columns (adhesiveness) induced by disturbing the blood.
    Proc Soc Exp Biol Med. 1971 Mar;136(3):769-71 PMID: 5555369
  4. Ristocetin--a new tool in the investigation of platelet aggregation.
    Thromb Diath Haemorrh. 1971 Oct 31;26(2):362-9 PMID: 5316292
  5. The isolation nd characterization of bovine factor VIII (antihemophilic factor).
    J Biol Chem. 1972 Apr 25;247(8):2512-21 PMID: 5019961
  6. Immunological characterization of purified anti-haemophilic factor A (factor VIII) which corrects abnormal platelet retention in Von Willebrand's disease.
    Nat New Biol. 1972 Mar 29;236(65):104-6 PMID: 4112547
  7. Immunologic studies of antihemophilic factor (AHF, factor VIII). IV. Radioimmunoassay of AHF antigen.
    J Lab Clin Med. 1972 Dec;80(6):822-33 PMID: 4634503
  8. Correction of the platelet abnormality in von Willebrand's disease by cryoprecipitate.
    Am J Med. 1972 Dec;53(6):734-8 PMID: 4539018
  9. Antigen-biological-activity ratio for factor VIII.
    Lancet. 1973 Mar 17;1(7803):616-7 PMID: 4120690
  10. Iodination of the human platelet membrane. Studies of the major surface glycoprotein.
    J Biol Chem. 1973 Apr 25;248(8):2928-36 PMID: 4697403
  11. Contractile proteins of endothelial cells, platelets and smooth muscle.
    Am J Pathol. 1973 Apr;71(1):1-22 PMID: 4267065
  12. Isolation and characterization of human Factor VIII (antihemophilic factor).
    J Biol Chem. 1973 Jun 10;248(11):3946-55 PMID: 4708094
  13. Interaction between platelets and von Willebrand factor provides a new scheme for primary haemostasis.
    Nat New Biol. 1973 Aug 1;244(135):159-60 PMID: 4541893
  14. Immunological studies of factor VIII in haemophilia and von Willebrand's disease.
    Nat New Biol. 1973 Aug 29;244(139):281-2 PMID: 4199570
  15. Defective ristocetin-induced platelet aggregation in von Willebrand's disease and its correction by factor VIII.
    J Clin Invest. 1973 Nov;52(11):2697-707 PMID: 4201262
  16. Quantitative assay of a plasma factor deficient in von Willebrand's disease that is necessary for platelet aggregation. Relationship to factor VIII procoagulant activity and antigen content.
    J Clin Invest. 1973 Nov;52(11):2708-16 PMID: 4542944
  17. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.
    J Clin Invest. 1973 Nov;52(11):2745-56 PMID: 4355998
  18. Synthesis of antihemophilic factor antigen by cultured human endothelial cells.
    J Clin Invest. 1973 Nov;52(11):2757-64 PMID: 4583980
  19. Von Willebrand factor: dissociation from antihemophilic factor procoagulant activity.
    Science. 1973 Dec 14;182(4117):1149-51 PMID: 4127287
  20. The specificity of antibodies to factor VIII produced in the rabbit after immunization with human cryoprecipitate.
    Thromb Diath Haemorrh. 1973 Jun 28;29(3):652-60 PMID: 4543423
  21. Studies on the nature of the circulating anticoagulant directed against antihemophilic factor: with notes on an assay for anthemophilic factor.
    Blood. 1962 Aug;20:137-49 PMID: 13872719
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.5.1906
Published
1974-05-00
Pages
1906-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388351
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.5.1906 PMC Full Text View journal details
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