Phagocytosis of live versus heat-killed bacteria by human polymorphonuclear leukocytes.

Infection and immunity ·Vol. 10 ·No. 1 ·1974-07-00 ·Pages 25-9

DeChatelet LR, Mullikin D, Shirley PS, McCall CE

Abstract

Heat-killed Pseudomonas aeruginosa are phagocytized much more slowly by human polymorphonuclear leukocytes than are the live organisms. The post-phagocytic increase in hexose monophosphate shunt activity (HMS) parallels the ingestion of the bacteria. The addition of serum to the live organisms causes a marked increase in both ingestion and cellular HMS activity; serum actually causes an inhibition of both uptake and HMS activity when added to the heat-killed organisms. Differences in postphagocytic HMS activity between live and heat-killed organisms were observed with three different species of bacteria, indicating that the phenomenon is not restricted to P. aeruginosa. These data emphasize that the influence of the particle on the phagocytic process is considerable.

MeSH Terms

Carbon Dioxide/metabolism Carbon Radioisotopes Glucose/metabolism Hexosephosphates/metabolism Hot Temperature Humans Leukocytes/immunology Phagocytosis Pseudomonas aeruginosa/immunology

Chemicals

Carbon Radioisotopes Hexosephosphates Carbon Dioxide Glucose

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

DeChatelet L R

Mullikin D

Shirley P S

McCall C E

References (9)

9 references, click to expand

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Influence of type of ingested particle on human leukocyte metabolism.
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Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function.
J Clin Invest. 1967 Sep;46(9):1422-32 PMID: 6036538
Effects of colchicine and chloramphenicol on the oxidative metabolism and phagocytic activity of human neutrophils.
J Infect Dis. 1973 Jan;127(1):40-8 PMID: 4566621
Effect of size and concentration of latex particles on respiration of human blood leucocytes.
Proc Soc Exp Biol Med. 1961 Aug-Sep;107:748-50 PMID: 14459576
Dissociation by colchicine of the hexose monophosphate shunt activation from the bactericidal activity of the leukocyte.
Infect Immun. 1971 Jan;3(1):66-72 PMID: 16557948
Complete deficiency of leukocyte glucose-6-phosphate dehydrogenase with defective bactericidal activity.
J Clin Invest. 1972 Apr;51(4):769-78 PMID: 4401271
The metabolism of leukocytes.
Semin Hematol. 1968 Apr;5(2):156-65 PMID: 5654923
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Proc Soc Exp Biol Med. 1972 Sep;140(4):1434-6 PMID: 5057606

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.10.1.25-29.1974

Published

1974-07-00

Pages

25-9

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC414951

Subset

External Links

PubMed source DOI: 10.1128/iai.10.1.25-29.1974 PMC Full Text View journal details

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Phagocytosis of live versus heat-killed bacteria by human polymorphonuclear leukocytes.

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