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PMID: 4212160 Published · ppublish English Journal Article

Messenger ribonucleic acid of dormant spores of Bacillus subtilis.

Journal of bacteriology ·Vol. 119 ·No. 2 ·1974-08-00 ·Pages 514-21

Jeng YH, Doi RH

Abstract

Evidence of the presence of messenger ribonucleic acid (mRNA) in dormant spores of Bacillus subtilis has been obtained. The bulk RNA from spores was isolated and labeled in vitro with tritiated dimethyl sulfate. The spore RNA hybridized to 2.4 to 3.2% of the B. subtilis genome. The RNA hybridized to both the complementary heavy and light fractions of deoxyribonucleic acid (DNA). Bulk RNA from log-phase cells competed with virtually all the spore RNA for the heavy DNA fraction and with part of the spore RNA for the light DNA fraction. Bulk RNA from stage IV cells in sporulation also competed with all of the spore RNA for the heavy DNA fraction and with essentially all the spore RNA for the light DNA fraction. These results indicate that dormant spores contain mRNA species present in both log-phase cells and stage IV cells of sporulation. The RNA polymerase in the developing forespore must be able to recognize promotor sites for both log-phase and sporulation genes.

MeSH Terms
Bacillus subtilis/analysis,enzymology DNA, Bacterial DNA-Directed RNA Polymerases/metabolism Methylation Nucleic Acid Hybridization RNA, Bacterial/analysis RNA, Messenger/analysis RNA, Ribosomal/analysis RNA, Transfer/analysis Spores, Bacterial/analysis Spores, Fungal/enzymology Sulfates Time Factors Tritium
Chemicals
DNA, Bacterial RNA, Bacterial RNA, Messenger RNA, Ribosomal Sulfates Tritium RNA, Transfer DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jeng Y H
Doi R H
References (35)
35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.119.2.514-521.1974
Published
1974-08-00
Pages
514-21
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245635
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.119.2.514-521.1974 PMC Full Text View journal details
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