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PMID: 4212281 Published · ppublish English Journal Article

Protein synthesis in Bacillus subtilis: differential effect of potassium ions on in vitro peptide chain initiation and elongation.

Journal of bacteriology ·Vol. 119 ·No. 3 ·1974-09-00 ·Pages 821-9

Sala F, Bazzicalupo M, Parisi B

Abstract

The preparation and fractionation of a highly active and stable in vitro protein-synthesizing system from Bacillus subtilis is described. Potassium satisfied the requirement for a monovalent ion when the initiation factor-dependent binding of formyl-methionyl-transfer ribonucleic acid and synthesis of formyl-methionyl-puromycin were assayed, whereas it inhibited the reactions for polyphenylalanine synthesis. On the other hand, the ammonium ion satisfied the requirement for all assayed reactions. The in vitro experimental evidence suggested that potassium is an inhibitor of one or a few specific reactions involved in peptide chain elongation in B. subtilis.

MeSH Terms
Bacillus subtilis/metabolism Bacterial Proteins/biosynthesis Carbon Radioisotopes Cell Fractionation Cell-Free System Escherichia coli Methionine/metabolism Peptide Biosynthesis Peptide Chain Elongation, Translational/drug effects Peptide Chain Initiation, Translational/drug effects Peptide Elongation Factors/metabolism Peptide Initiation Factors/metabolism Phenylalanine/metabolism Potassium/drug effects Puromycin/biosynthesis RNA, Transfer/metabolism Ribosomes/metabolism
Chemicals
Bacterial Proteins Carbon Radioisotopes Peptide Elongation Factors Peptide Initiation Factors Phenylalanine Puromycin RNA, Transfer Methionine Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sala F
Bazzicalupo M
Parisi B
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.119.3.821-829.1974
Published
1974-09-00
Pages
821-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245686
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.119.3.821-829.1974 PMC Full Text View journal details
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