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PMID: 4215081 Published · ppublish English Journal Article

The localization of transfer RNA5Lys genes in Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3527-31

Grigliatti TA, White BN, Tener GM, Kaufman TC, Suzuki DT

Abstract

Transfer RNA(5) (Lys) was isolated and purified from Drosophila melanogaster. It was iodinated in vitro with sodium [(125)I]iodide and hybridized in situ to the polytene salivary gland chromosomes from Drosophila. Autoradiographs exposed three to four weeks allowed the localization of this tRNA to the right arm of chromosome 2 between bands 48F and 49A.

MeSH Terms
Chromatography, DEAE-Cellulose Chromosome Mapping Drosophila melanogaster/metabolism Electrophoresis, Polyacrylamide Gel Genes Iodine Radioisotopes Lysine Nucleic Acid Hybridization RNA, Bacterial/isolation & purification,metabolism RNA, Transfer/isolation & purification,metabolism Salivary Glands/ultrastructure Transcription, Genetic
Chemicals
Iodine Radioisotopes RNA, Bacterial RNA, Transfer Lysine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Grigliatti T A
White B N
Tener G M
Kaufman T C
Suzuki D T
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.9.3527
Published
1974-09-00
Pages
3527-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433807
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.9.3527 PMC Full Text View journal details
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